摘要
分别采取高温灭菌和滤菌器过滤2种方式,对韭菜、大蒜、大葱、洋葱等4种葱科植物的组织粗提液进行除菌,并用其进行香蕉枯萎病病原菌(Fusarium oxysporum f.sp.cubense)的拮抗实验。结果表明:滤菌器过滤处理组比高温灭菌处理组对香蕉枯萎病菌的抑制效果更显著;在滤菌器除菌组中,韭菜的抑菌效果最佳,其次为大蒜和大葱,与对照相比都达到了差异极显著水平,但洋葱粗提液的抑菌效果不明显。
Four Alliaceae plants including Chinese chives (Allium tuberosum),garlic (Allium sativum),green Chinese onion (Alliumfistulosum),and onion (Allium cepa) were selected and extracted for antagonistic experiments to observe the antagonistic activities of their crude extracts against Fusarium oxysporum f.sp.cubense tropical race 4 (Foc TR4).These crude extracts were isolated and sterilized by using the high temperature and the bacteria-proof filter methods,respectively.The crude extracts sterilized by the bacteria-proof filter method were found to have obviously higher inhibition of the Foc TR4 than those by the high temperature method.The crude extract from Chinese chives showed the best inhibitory effect on the Foc TR4,followed by those from garlic and green Chinese onion,and they all showed inhibitory effect on the Foc TR4 at a very significant level as against the control.However,the crude extract from onion showed no significant inhibitory effect on the Foc TR4.
出处
《热带生物学报》
2014年第3期239-243,共5页
Journal of Tropical Biology
基金
国家自然科学基金资助项目(31360364)
教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(lhxm-2012-2)
海南省重大科技项目(ZDZX2013023)
"中央财政支持中西部高校提升综合实力专项"子课题
关键词
香蕉枯萎病
尖孢镰刀菌古巴专化型
葱科植物
植物粗提液
抑菌效果
banana fusarium wilt
Fusarium oxysporum f.sp.cubense
Alliaceae plants
plant crude extracts
inhibitory effect