摘要
以菌体生长对数末期发酵液OD600值为优化标准,通过单因素和正交试验方法对前期室内实验筛选得到的对棉铃虫(Helicoverpa armigera Hubner)有较高活性的苏云金芽孢杆菌Bt A322菌株进行培养基优化,并进行发酵。结果表明,最佳培养基配方为0.5%黄豆粉+0.5%酵母粉+0.5%葡萄糖+0.5%胰蛋白胨+0.2%碳酸钙+0.1%磷酸二氢钾+0.1%硫酸镁。最佳培养条件为培养时间20 h,初始p H值7.5,发酵温度30℃,摇床转速为200 r·min-1,接种量3%,250 m L三角瓶适宜装35 m L培养基。优化后的菌体生长量OD600值比优化前的提高了44.7%。
Bacillus thuringiensis Berliner strain A322 with higher activity against Helicoverpa armigera Hubner screened indoor was cultured on media through single-factor and orthogonal experiment to optimize cultural medium based on the optimization standard of OD600 of the fermented liquid of B.thuringiensis at the late logarithmic phase.The optimum medium is 0.5% soybean meal,0.5% yeast extract,0.5% glucose,0.5% tryptone,0.2% calcium carbonate,0.1% potassium dihydrogen phosphate,and 0.1% magnesium sulfate.The major factors in laboratory shake flask liquid fermentation,such as initial pH,temperature,speed,etc were determined.The optimum cultural conditions are found to be incubation time 20 h,pH7.5,fermentation temperatures 30 ℃,speed 200 r · min^-1,inoculum size 3%,and medium volume 35 mL in a 250 mL flask.The OD600 value of the bacterial growth was increased by 21.9% after optimization of the cultural conditions.
出处
《热带生物学报》
2014年第3期253-259,共7页
Journal of Tropical Biology
基金
海南大学"中西部高校提升综合实力专项资金项目"
海南大学热带作物种质资源保护与开发利用教育部重点实验室资助项目