丹参磷脂浸膏对急性四氯化碳肝损伤小鼠肝组织的保护作用

Protective effect of DSLZ on acute liver injury induced by CCl_4 in mice

目的探讨丹参磷脂浸膏(DSLZ)对实验小鼠肝损伤的保护作用。方法 ICR小鼠60只,随机分为空白对照组、模型组及丹参磷脂浸膏高、中、低剂量组和阳性对照丹参酮IIA(SalviolⅡA IIA)组,采用四氯化碳(CCl4)法建立急性肝损伤实验动物模型,观察丹参磷脂浸膏对实验小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、丙二醛(MDA)、还原型谷胱甘肽(GSH)以及肝组织病理改变的影响。结果与空白对照组比较,模型组小鼠血清ALT、AST及MDA均显著增高(P<0.01),GSH明显降低(P<0.01)。丹参磷脂浸膏高、中剂量组ALT、AST及MDA明显降低(P<0.05或P<0.01),GSH明显升高(P<0.05或P<0.01)。HE染色显示丹参磷脂浸膏组小鼠肝组织病理损伤减轻。结论丹参磷脂浸膏对小鼠急性四氯化碳性肝损伤具有明显的抑制作用,其机制可能与抗氧化有关。

Objective To explore the protective effect of DSLZ on acute chemical liver injury induced by 1% carbon Tetrachloride（CCl4）in mice and to reveal its mechanism.Methods 60 mice were randomly divided into normal control group,model group,SalviolⅡ A group,high dose DSLZ group（2.6mg/kg）,middle dose group（1.56mg/kg）and low dose group（0.52mg/kg）.All the mice were intragastrically administered the corresponding agents respectively for 30 days.After the last administration 1% CCl42.0ml/kg was given to mice in all groups except control group in a single administration of vegetable oil intragastrically to establish the acute liver injury mouse model.16 hlater,the mice were sacrificed,alanine aminotransferase（ALT）,aspartate aminotrsferase（AST）,malondialdehyde（MDA）,reduced glutathione hormone（GSH）level in serum were detected.HE staining was performed for observing the pathological changes of the liver.Results Compared with the control group,the level of ALT,AST,MDA in the serum of the model group mice were increased significantly,and the GSH level was decreased significantly（P〈0.01）.Compared with model group,the serum ALT,AST,MDA level in hign dose of DSLZ and middle dose of DSLZ groups were decreased significantly（P〈0.05 or P〈0.01）,while the GSH level in serum were increased significantly（P〈0.05 or P〈0.01）.Moreover the HE staining results showed that in model group the swelling,kytoplasm coacervation,hydropic degeneration and necrosis of liver cells were seen.The morphological damage of liver cells were improved significantly in DSLZ groups.Conclusion DSLZ has a significant protective effect on CCl4–induced acute liver injury,which might be related to the anti-oxidation.