摘要
目的研究评价胰腺癌和胰腺炎患者血清胸苷激酶1(TK1)的表达,同时检测胰腺癌肿瘤标记物糖类抗原19-9(CA19-9)和癌胚抗原(CEA),探讨联合检测TK1、CA19-9和CEA在胰腺癌与胰腺炎鉴别诊断方面的临床价值。方法应用化学增强发光(ECL)点印迹法定量检测43例胰腺癌和40例胰腺炎患者血清TK1浓度,直接化学发光法检测血清CEA和CA19-9浓度;50例健康志愿者作为对照组。结果血清CA19-9、CEA水平胰腺癌组、胰腺炎组明显高于对照组(P均<0.05),但胰腺癌组与胰腺炎组比较差异无统计学意义。血清TK1水平胰腺癌组明显高于胰腺炎组、对照组(P均<0.05),而胰腺炎组与对照组比较差异无统计学意义。结论联合检测血清CA199、CEA和TK1有助于胰腺炎和胰腺癌的鉴别诊断。
Objective Thymidine kinase 1in serum（TK1）has been found to be a reliable proliferation marker in clinical trials.In this study,we examined the significance of combined detection of serum tumor marker TK1,CAl9-9and CEA in pancreatic cancer and pancreatitis patients.Methods The concentrations of CA19-9and CEA were measured by automatic chemiluminescence immunoassay.That of TK1 was measured by a sensitive dot blot ECL assay.Results Compared with the 50 healthy persons control group,the concentrations of CAl9-9and CEA were higher both in43 patients with pancreatic cancer and 40 patients with pancreatitis（P〈0.05）.But the concentration of TK1 was higher in patients with pancreatic cancer only（P〈0.05）.Conclusion The combined detection of serum TK1,CA19-9and CEA is valuable to the differential diagnosis of pancreatic cancer and pancreatitis.
出处
《中国实验诊断学》
2014年第11期1773-1776,共4页
Chinese Journal of Laboratory Diagnosis
