向日葵籽壳湿氧化预处理与同步糖化发酵制生物乙醇

Preparation of bioethanol from wet oxidized sunflower seed shell by simultaneous saccharification and fermentation

以食用型向日葵籽壳为原料,采用湿氧化预处理和同步糖化发酵法制备生物乙醇,并通过正交实验确定了二者最佳工艺条件。结果表明,前者最佳工艺条件为:反应温度190℃,氧气压强1.2 MPa,过筛干燥后的向日葵籽壳/混合溶液(质量体积比)1∶10,时间15 min;后者最佳工艺条件为:反应温度40℃,纤维素酶用量30 U/g,p H值4.8,酵母接种量8%。在此条件下,纤维素转化率为83.66%,光密度为0.995,含糖量为243.3 mg,生物乙醇收率为18.04%。

The bioethanol was preparaed from sunflow- er seed shell by wet oxidation pretreatment and simulta- neous saccharification and fermentation （SSF）, and the best process conditions were confirmed by orthogonal tests. The results showed that the optimum process condition for wet oxidation was reaction temperature 190 ℃ , oxygen pressure 1.2 MPa, pretreated sun- flower seed shell/hydrolysis mixed solution （ratio of mass to volume） 1： 10, pretreatment time 15 min.The optimum condition for the SSF was reaction temperature 40 ℃ , cellulase amount 30 U/g, pH value 4.8, yeast inoculums 8%. Under these conditions, the cellulose conversion was 83.66% ,optical density value 0. 995, sugar content 243.3 mg, and the yield of bioethanol nearly 18.04%.