摘要
采用脂肪酶Lipozyme RM IM催化辛酸、癸酸与大豆油进行酸解反应以制备MLM型结构脂质。通过单因素实验研究底物摩尔比、辛癸酸摩尔比、酶添加量、反应时间、反应温度和初始水分含量对酸解反应的影响。得到适合的反应条件为:底物摩尔比3∶1(总脂肪酸/大豆油),辛酸与癸酸摩尔比(辛酸/癸酸)2.5∶1,酶添加量7.5 wt%(基于底物总重),反应时间5 h,反应温度65℃,加水量1.0 wt%(基于底物总重),得到MLM结构脂脂肪酸组成中辛酸含量为20.0 wt%,癸酸含量为10.5 wt%,两者质量比为1.92。
The acidic hydrolysis of soybean oil with caprylic acid and capric acid was utilized to manufacture MLM–type structured lipids using Lipozyme RM IM as the catalyst. Single factor experiments were applied to study the effects of substrates molar ratio,molar ratio of caprylic acid to capric acid,enzyme load,reaction time,reaction temperature and initial water content,giving the following optimum reaction conditions：substrate mole ratio 3∶1（total fatty acid/soybean oil）,molar ratio of caprylic acid to capric acid 2.5∶1,enzyme load 7.5 wt%（of the substrate mass）,5 h,65℃,and initial water content 1 wt%（of the substrate mass）. The production contains caprylic acid 20.0 wt%, capric acid 10.5 wt%,and their mass ratio is 1.92.
出处
《粮食与油脂》
北大核心
2014年第11期39-44,共6页
Cereals & Oils
基金
广东省战略性新兴产业核心技术攻关项目(2012A080800013)
