甲基磺酸乙酯诱变杜梨种子突变的鉴定与分析

Identification and analysis of EMS-induced seed mutants in Pyrus betulifolia

为了获得杜梨Pyrus betulifolia突变体,以杜梨种子为试材,分别使用不同体积分数甲基磺酸乙酯(EMS)[0(ck),0.1%,0.2%,0.3%,0.4%,0.5%,0.6%,0.7%,0.8%,0.9%]对层积后的种子进行诱变处理。然后,统计了种子发芽率,使用相关序列扩增多态性(SRAP)标记鉴定遗传变异位点,测量幼苗田间株高和节间长度。试验结果表明:杜梨种子发芽的半致死剂量为0.3%EMS处理。结合SRAP标记鉴定和表型数据分析,筛选出最适宜的EMS处理体积分数为0.4%,变异率为37.3%;同时,使用SPSS软件对表型数据分析显示,处理组的杜梨苗株高极显著低于对照组(P=0.01)。研究结果为快速获得杜梨突变体提供了理论基础和依据。

To obtaining the mutants of Pyrus betulifolia,different concentrations （0,0.1％,0.2％,0.3％,0.4％,0.5％,0.6％,0.7％,0.8％,0.9％） of ethyl methane sulphonate（EMS） were used to induce seeds after stratification.Then,seed germination percentage was determined,sequence-related amplified polymorphism （SRAP） markers were used to identify mutant loci,and phenotypic measurements of plant height and internode length of the seedlings were made.Results showed that the half lethal dose of EMS concentration was 0.3％.Combined with SRAP markers detection and phenotypic data analysis,the optimum concentration of EMS treatment was 0.4％,and the mutation rate was 37.3％.Meanwhile,the phenotypic data analysis which used SPSS revealed that plant height with different EMS treatments was significantly lower （P =0.01） than the ck group.These results should provide a valuable theoretical basis for effectively obtaining pear mutants.