松节油对米尔贝霉素的杀螨增效活性及稳定性

Acaricidal activity and stability of milbemycins used in turpentine

为明确松节油对米尔贝霉素的增效活性和稳定性,采用叶碟喷雾法,分别测定了以松节油和乙醇为溶剂配制的米尔贝霉素乳油对朱砂叶螨Tetranychus cinnabarinus的毒力,半致死质量浓度(CL50)分别为0.031 4,0.139 0mg·L-1,共毒系数为442.68,表现出显著的增效活性。光解试验表明,以松节油为溶剂能够提高米尔贝霉素的稳定性。气相色谱-质谱联用仪(GC-MS)分析,松节油中α-蒎烯、莰烯、(R)-苧烯、3-蒈烯等萜烯类物质总含量超过60%,萜烯类物质对昆虫表皮具有良好的渗透作用,是其增效作用的主要原因。松节油可作为溶剂和增效剂用于米尔贝霉素绿色乳油制剂加工。

To verify synergistic activity and stability of milbemycin in turpentine,turpentine oil and ethanol with a solvent mixture of milbemycin were measured.Virulence was tested on the carmine spider mite （Tetranychus cinnabarinus） using a leaf disc spray.A photolysis experiment was conducted to determine the stability of milbemycin,and a Gas Chromatography-Mass Spectrography （GC-MS） analysis was performed.Results showed that toxicities of different milbemycin oil in lethal concentration 50 （LC50） values on female adult mites were 0.031 4 and 0.139 0 mg·L^-1 with a poison coefficient of 442.68 reflecting a strong synergistic activity.The photolysis experiment showed that turpentine oil as a solvent could improve the stability of milbemycins.The GC-MS analysis revealed that the content of alpha-pinene,camphene,R-limonene and 3-carene was over 60%.Since the main reason for synergistic activity was favorable osmosis of terpene chemicals entering the insect cuticle,turpentine could be used as a solvent and synergistic agent for processing milbemycin green oil preparations.