慢性牙周炎患者治疗前后患牙龈沟液中T细胞亚群表达水平的研究

Levels of T Cell subsets in gingival crevicular fluid in chronic periodontitis patients before and after treatment

目的探讨慢性牙周炎患者治疗前后龈沟液(GCF)中T细胞亚群中CD3+、CD4+、CD8+、CD4+/CD8+的改变及其临床意义。方法选取我院进行治疗的48例慢性牙周炎患者为治疗组,全部常规治疗,正常健康对照组32例,检测治疗组治疗前、治疗后6、12、24周的临床指数,采用流式细胞术检测治疗组治疗前后及正常对照组GCF中CD3+、CD4+、CD8+的所占比例及计算CD4+/CD8+的值,并分析二者的相关性。结果慢性牙周炎通过常规治疗后,牙周临床指标包括PD、CAL、PI和PLI均有明显改善(P<0.01或P<0.05);治疗组GCF中CD3+、CD4+、CD8+和CD4+/CD8+在治疗前和治疗后的6周、12周与对照组的相应时间点比较,差异有统计学意义(P<0.01);治疗前水平与治疗后6周、12周和24周比较,差异有统计学意义(P<0.01);治疗前后PD、PI和PLI与GCF中T细胞亚群的水平有明显相关性(P<0.01或P<0.05)。结论 T淋巴细胞亚群的变化与慢性牙周炎的炎症状态密切相关,可作为一项评估预后的指标。

Objective To investigate the changes in levels ofT cell subsets CD3^＋, CD4^＋, CD8^＋ and CD4^＋/CD8^＋ in gingival crcvicular fluid （GCF） in chronic periodontitis patients before and after treatment and their clinical significance. Methods The treatment group included 48 patients with chronic pedodontitis who were given conventional periodontal treatment, while the control group enrolled 32 pcriodontally healthy individuals. Clinical parameters were collected at baseline and 6, 12 and 24 weeks after treatment, along with GCF CD3^＋, CD4^＋, CD8^＋ levels and CD4^＋/CD8^＋ ratios, which was measured using flow cytometry. The correlation between clinical parameters and T cell subset levels was analyzed. Results Significant improvement of clinical parameters in chronic periodontitis patients was observed following conventional treatment （P〈0.01 or P〈0.05）. There were statistical differences between the treatment and control groups in CD3＋, CD4＋, CD8＋ and CD4＋/CD8＋ levels at baseline, and 6 and 12 weeks afler treatment （P〈0.01）. There were statistical differences between baseline levels and levels at 6, 12 and 24 weeks after treatment （P〈0.01）. The correlation between clinical parameters, including PD, PI and PLI, and GCF T cell subset levels before and after treatment, was statistically significant （P〈0.05 or P〈0.01）. Conclusion GCF T cell subset levels are closely associated with the clinical inflammatory status of chronic periodontitis and may be used as markers for evaluation of prognosis.