静脉留置针致血栓形成与vWF、DD浓度关系的动物实验研究

Animal study of thrombosis detaining needle and changes of vWF and DD

目的 探讨静脉留置针不同留置时间静脉血栓形成与vWF、DD浓度变化的关系,寻找早期预测静脉血栓形成的敏感指标,为选择干预时机提供理论和实验依据.方法 选择36只实验兔随机分为甘露醇组和生理盐水组各18只,在兔耳缘静脉置入留置针并留置输液,分别测定甘露醇组和生理盐水组不同留置时间vWF和DD的浓度变化,同时病理学观察置针血管静脉血栓形成情况.结果 血清学检测结果：（1）vWF浓度：置针1d时,2组vWF浓度达到高峰.（2）DD浓度：置针后2组DD浓度均高于置针前,其中置针1、3d时DD浓度与置针前比较差异有统计学意义.病理学观察结果：置针1d时,2组静脉血栓形成率达100％,血栓程度为中度以上.结论 vWF和DD浓度的测定对预测留置针致静脉血栓形成有一定的参考价值,静脉留置针置入后应尽早进行干预,防止静脉血栓形成.

Objective To observe the relations ot vWF,DD concentration and venous thrombosis caused by detaining needle of different time.Sensitive indicators for early prediction of venous thrombosis would be seeked so as to provide theoretical and experimental basis for the selection of intervention time.Methods The detaining needle were placed in the vein of rabbit ears with transfusion.The changes of vWF and DD concentration for the mannitol group and the saline group were measured in different retention time,while the venous thrombosis caused by detaining needle was observed pathologically.Results The results of serological test：（1）vWF concentration：when the needle was detained for 1 day,the vWF concentration of two groups peaked.（2）DD concentration：after the needler was set,the DD concentration of both groups was higher than those before detaining needler.When the needle was detained for 1 day and 3 days,the DD concentration was significantly different from those before using detaining needle.Result of pathological observation：When the needle was detained for 1 day,the venous throm-bosis rate was 100% for both groups with a moderate degree of thrombosis.Conclusions Detection of the concentration of vWF and DD has certain reference value for predicting detaining needle-induced venous thrombosis.In order to prevent venous thrombosis,intervention should be carried out as soon as possible after detaining needle was set.