直接熔融缩聚法合成PLGA的表征及其生物相容性研究

Synthesis and Characterization of PLGA by Melt Polycondensation

本文旨在探究一条更加适合用于医学研究的高分子材料PLGA的合成途径。首先在无催化剂、高真空条件下直接熔融缩聚合成聚乳酸-聚乙醇酸（PLGA）的无规则共聚物，对其合成反应体系的最佳温度范围、反应时间进行确定，然后对反应产物的分子量、结构、亲水性能、热性能以及不同比例LA/GA的产物特性粘数等物化性质进行表征，对合成的高分子材料PLGA的生物相容性进行分析与讨论。结果显示在无催化剂、高真空、160~175℃、LA/GA的比例为3∶7左右、反应时间60 h左右下合成的高分子材料PLGA具有较稳定的物化性质和较好的生物相容性，可以作为以后用于医学研究的高分子材料PLGA的合成途径。

Purpose This study is to explore a more suitable biosynthetic pathway of the polymer materials PLGA which is used in medical research. Method First of all , reference relevant literature direct melt polycondensation poly lactic acid- glycolic acid （PLGA） of random copolymer under the absence of catalyst and high vacuum condition , Through the relevant method determine the reaction time and the optimal temperature range of the reaction system, Then the molecular weight of the reaction products, structure and hydrophilic properties of the different proportions of LA/GA product of the comparison of intrinsic viscosity, the synthesis of PLGA and PGA thermal performance comparison of physicochemical properties are characterized. Also, biocompatibility of synthetic polymer material PLGA with fibroblasts are discussed. Conclusion Our experiment shows that the synthetic polymer materials PLGA has a more stable physical and chemical properties and good biocompatibility in the absence of catalyst, high vacuum, 160~175 ＆#176; C, LA/GA ratio of about 3：7, reaction time around 60h condition. Therefore, this method can be used as the biosynthetic pathway of the PLGA polymer materials used in medical research.