幽门螺杆菌感染与口腔扁平苔藓和鳞状细胞癌相关性的初步探讨

A preliminary study on the correlation between the Helicobacter pylori infection and oral lichen planus and squamous cell carcinoma

目的研究正常口腔黏膜、口腔扁平苔藓(OLP)和口腔鳞状细胞癌(OSCC)中幽门螺杆菌(H.pylori)感染情况及其与p53蛋白和p16蛋白表达的关系,探讨H.pylori感染与OLP和OSCC的相关性。方法 OLP标本23例、OSCC标本50例和正常口腔黏膜标本10例,采用免疫组化SP法检测其H.pylori的感染、p53蛋白、p16蛋白的表达,应用SPSS 13.0软件包对结果进行卡方分析检验,单因素方差分析和线性回归检验分析。结果正常口腔黏膜组织组、OLP组及OSCC组中H.pylori阳性率分别为0、47.8%和48.0%,正常口腔黏膜组与OLP组和OSCC组间差异有统计学意义(χ2=7.17,P=0.013;χ2=8.00,P=0.004);OLP标本中H.pylori阳性组和阴性组p53蛋白表达率分别为45.5%和16.7%,p16蛋白表达率为分别36.4%和66.7%,组间差异有统计学意义(χ2=3.86,P=0.043;χ2=3.89,P=0.048);OSCC标本中H.pylori阳性组和阴性组p53蛋白表达率分别为91.7%和84.6%,p16蛋白表达率为分别20.8%和30.8%,组间差异无统计学意义(χ2=0.58,P=0.669;χ2=0.65,P=0.526)。结论 H.pylori感染与OLP和OSCC存在一定的相关性,H.pylori感染在OLP和OSCC发生、发展中的作用有待于进一步研究。

Objective To investigate the correlation between the Helicobacter pylori（H.pylori） infection and oral lichen planus （OLP） and squamous cell carcinoma （OSCC）, the H.pylori infection, expressions of p53 and p16 in the normal oral mucosa, oral lichen planus and squamous cell carcinoma were detected. Methods Twenty-three oral lichen planus, 50 squamous cell carcinoma and 10 normal oral mucosa were tested. The H.pylori infection and the expressions of p53 and p16 were detected by immunohistochemical technique （SP method）. The data was analyzed by Fisher＇s Exact Test, one-way ANOVA and linear regression analysis using SPSS 13.0 software package. Results The rate of H.pylori infection in normal oral mucosa, oral lichen planus and squamous cell carcinoma were 0%, 47.8% and 48.0% respectively. There were significant differences between the normal oral mucosa and the oral lichen planus and squamous cell carcinoma （P〈 0.05）. The rate of p53 expression in the H.pylori positive and negative of oral lichen planus were 45.5% and 16.7%, and the p16 were 36.4% and 66.7% respectively. There were significant differences between the groups （P〈 0.05）. The rate of p53 expression in the H.pylori positive and negative of oral squamous cell carcinoma were 91.7% and 84.6%, and the p16 were 20.8% and 30.8% respectively. There were no significant differences between the groups （P〉 0.05）. Conclusions There was the correlation between the the H.pylori infection and oral lichen planus and squamous cell carcinoma. The effect of H.pylori infection on the occurrence and development of oral lichen planus and squamous cell carcinoma is needed more explorsion.