类风湿关节炎患者外周血髓源性抑制细胞的检测及其与Th1/Th2细胞的相关性

Detection of peripheral blood myeloid derived suppressor cells in patients with rheumatoid arthritis and its correlation with Th1/Th2 cells

目的检测类风湿关节炎患者外周血髓源性抑制细胞和Th1以及Th2,并分析髓源性抑制细胞与Th1/Th2细胞的相关性。方法选取湖南省永州市中心医院2012年1月至2013年12月的40例类风湿关节炎患者为研究组,将其分为活动组和稳定组两个亚组,并且选取同期在湖南省永州市中心医院健康体检的40例健康者为健康对照组,测定两组研究对象的外周血髓源性抑制细胞和Th1、Th2细胞,并分析髓源性抑制细胞与Th1/Th2细胞的相关性。结果研究组患者髓源性抑制细胞和Th1、Th2细胞的百分比均明显高于健康对照组,且两组比较差异有统计学意义(P<0.05)。活动组中的髓源性抑制细胞和Th1细胞的百分比明显高于稳定组,而Th2细胞百分比明显低于稳定组,差异有统计学意义(P<0.05)。活动组和稳定组的髓源性抑制细胞和Th1细胞的百分比均高于健康对照组,而活动组与健康对照组Th2细胞百分比比较,差异无统计学意义(P>0.05);稳定组Th2细胞百分比高于健康对照组,差异统计学有意义(P<0.05)。髓源性抑制细胞百分比与Th1细胞百分比呈现负相关性(P<0.05),而与Th2细胞百分比无相关性,差异无统计学意义(P>0.05)。结论临床中类风湿关节炎患者外周血中的髓源性抑制细胞呈现高表达,且与Th1细胞百分比呈现负相关性,而与Th2细胞百分比无相关性。提示外周血中的髓源性抑制细胞和Th1细胞可能呈相互拮抗作用,并且共同参与类风湿关节炎的发生、发展。

Objective To detect the peripheral blood myeloid derived suppressor cells and Th1 and Th2 in the patients with rheumatoid arthritis ,and to analyze the correlation between myeloid derived suppressor cells and Th1/Th2 cells .Methods 40 cases of rheumatoid arthritis in the Yongzhou Municipal Central Hospital from January 2012 to December 2013 were selected as the study group and divided into the active subgroup and the stable subgroup . Contemporaneous 40 individuals with the health physical examination were selected as the healthy control group .The peripheral blood myeloid derived suppressor cells ,Th1 and Th2 cells were detected in the two groups and the correla‐tion between myeloid derived suppressor cells and Th1/Th2 cells was analyzed .Results The percentages of myeloid derived suppressor cells ,Th1 cells and Th2 cells in the study group were significantly higher than those in the healthy control group with statistically significant difference between the two groups（P〈0 .05） .The percentages of myeloid derived suppressor cells and Th1 cells in the active subgroup were significantly higher than those in the stable sub‐group and the percentage of Th2 cells was significantly lower than that in the stable subgroup ,the difference was sta‐tistically significant（P〈0 .05） .The percentages of myeloid derived suppressor cells and Th1 cells in the active sub‐group and the stable subgroup were significantly higher than those in the healthy control group ,but the percentage of Th2 cells had no statistical difference between the active subgroup and the healthy control group（P〉0 .05）;the Th2 cell percentage in the stable subgroup was higher than that in the healthy control group ,the difference had statistical significance（P〈0 .05） .The percentage of myeloid derived suppressor cells was negatively correlated with the per‐centage of Th1 cells（P〈0 .05） ,but had no correlation with the percentage of Th2 cells ,the difference had no statisti‐cal significance（P〉0 .05） .Conclusion The peripheral blood myeloid derived suppressor cells are highly expressed in the patients with rheumatoid arthritis in clinic ,negatively correlated with the percentage of Th1 cells and have no cor‐relation with the percentage of Th2 cells ,which prompts that peripheral blood myeloid derived suppressor cells and Th1 cells may have the mutual antagonistic effect and jointly participate in the occurrence and development of rheu‐matoid arthritis .