大鼠DAI特殊染色观察及NF-κB的表达

Special Staining Pathologic Changes and the Expression of NF-κB After Diffuse Axonal Injury in Rats

为了分析大鼠弥漫性轴索损伤(DAI)后病理学变化及核因子-κB(NF-κB)的表达,探讨了NF-κB在弥漫性轴索损伤中的作用,构建了大鼠DAI动物模型.运用Glees-Marsland染色法和Weil氏染色法观察伤后不同时间段的病理学改变,并运用免疫组化SABC法观察NF-κB的表达.实验结果表明,大鼠弥漫性轴索损伤后1h即可观察到部分轴索肿胀、断裂,髓鞘水肿、分层等现象,24h可见典型轴索收缩球形成,髓鞘崩解呈筛网状改变,72h轴索断裂数量、范围及髓鞘筛网状改变达到高峰;伤后1h,脑组织内可见NF-κB阳性细胞,伤后3h阳性细胞数增多,伤后24h达到高峰,持续到72h,伤后7d,仍可见阳性细胞表达,但数量减少.对照组细胞核未见阳性表达.大鼠弥漫性轴索损伤后NF-κB表达增加,可能与轴索继发性损伤有关.

To observe the pathologic changes and the expression of nuclear factor-kappa B（NF-κB）after diffuse axonal injury（DAD in rats,its mechanism is firstly explored. The rat DAI model was established. Immunohistochemical SABC method was used to observe the expression of NF-κB, combining Glees- Marsland and Well staining to observe the pathologic changes in different stage of DAI. Swollen and ruptured axon were found in brain tissue 1 h after DAI, and myelin sheath showed edema, delamination, marked axonal retraction ball and screen shape changes were detected after 24 h,and those morphological changes reached the peak after 72 h. The expression of NF-κB could be detected 1h after injury,the positive cells increased after 3 h, peaked after 24 h, and kept this tendency for 72 h. The positive cells decreased after 7 d. The control group showed negative staining. The findings showed that the expression of NF-κB increased in rat DAI model. The NF-κB may play an important role in secondary axonal injury.