RASSF2基因甲基化与卵巢透明细胞癌的相关性

Investigation on the Correlation between Hypermethylation of RASSF2 and Clear Cell Ovarian Carcinoma

目的探讨RASSF2基因异常甲基化与卵巢透明细胞癌的相关性。方法分别通过甲基化特异性PCR技术及免疫组化技术检测48例卵巢透明细胞癌组织中RASSF2基因的甲基化及蛋白表达情况。结果透明细胞癌组织中RASSF2基因的甲基化发生率(45.83%,22/48)及蛋白表达缺失发生率(47.92%,23/48)均显著高于正常卵巢组织及癌旁异位内膜组织,差异有统计学意义(P<0.05)。RASSF2基因甲基化与其蛋白表达缺失具有相关性。结论 RASSF2基因异常甲基化所导致的蛋白表达缺失可能在卵巢透明细胞癌的发病机制中具有重要作用。

Objective To investigate the correlation between hypermethylation of RASSF2 and ovarian clear cell carcinoma.Methods Methylation-specific PCR and immunohistochemistry were performed in 48 ovarian clear cell carcinoma samples to detect the methylation status and protein expression of RASSF2,respectively.Results In the ovarian clear cell carcinoma tissue,methylation frequency （45.83％,22/48） and absence of protein expression of RASSF2 （47.92％,23/48） was higher than that in both normal ovarian tissue and ectopic endometria adjacent to the cancerous region,respectively （P ＜ 0.05）.Absence of protein expression of RASSF2 was significantly correlated with the hypermethylation of the gene.Condusion Epigenetic inactivation of RASSF2 by promoter hypermethylation may be associated with the pathogenesis of clear cell ovarian carcinoma.