上、中、下部位鹿茸对PC12细胞促增殖和诱导分化的影响

Proliferation-Promoting and Differentiation-Inducing Effects of Different Sections of Velvet Antler on PC12 Cells

目的:探讨鲜品鹿茸上、中、下部位对PC12细胞的促增殖和诱导分化作用。方法:采用BCA试剂盒法测定样品的蛋白浓度;以大鼠肾上腺嗜铬细胞瘤株PC12细胞为研究模型,以MTT法检测鲜鹿茸上、中、下不同部位,不同浓度(5、10、50、100、200、300、500、800 mg·m L-1)的水提液对PC12细胞增殖活性的影响;显微观察计数法评价上、中、下不同部位,不同浓度的鲜鹿茸对细胞的促分化能力,统计分析细胞分化率。结果:鹿茸上、中、下部位可溶性蛋白含量分别为13.18、12.16和9.08 mg·m L-1;所有样品对细胞增殖均有促进作用,且呈现浓度效应。鹿茸上部促增殖能力最强,中部其次,下部最低。上部高浓度样品的促增殖率可达到60%,中部与上部样品的作用效果在高浓度(大于等于300 mg·m L-1)时差异不显著。鹿茸诱导细胞分化的能力,上部最强,中部和下部依次降低。随样品浓度增加,细胞分化率增加,当浓度为800 mg·m L-1时,上、中、下部位对细胞的分化率分别为91.38%、80.29%、76.52%。中部与下部样品对细胞分化程度的影响相差不大。

Objective： To explore the proliferation-promoting and differentiation-inducing effects of the different sections of the velvet antler on rat adrenal pheochromocytoma PC12 cells. Method： The rat adrenal pheochroocytoma PC12 was used as the cell model. The fresh velvet antler was divided into upper,middle and lower sections according to the sample＇s ossification degrees. The fresh samples were frozen homogenized to obtain the crude water extracts. The soluble protein content of the crude extracts was assayed by BCA kit. The cell proliferation rates were measured of different concentrations（ 5,10,50,100,200,300,500,800 mg·m L- 1） by the MTT method. PC 12 cells differentiation levels were observed under a microscopic and the differentiated cells were counted. Results： The protein contents for the upper,middle and lower sections were 13. 18,12. 16 and 9. 08 mg·m L- 1separately. The data showed that all the upper,middle and lower sections of the velvet antler crude extracts had significant cell proliferation promoting activities. The promoting effects were dose-dependent. The upper section had the strongest activity,followed by the middle and the lower section. The cells proliferation promoting rates increased 60% after treated with the high concentration of upper sample. There was no significant difference between the high concentrations（ ≥300 mg·m L^-1） and the middle and the upper samples. The upper sections samples showed the strongest cell differentiation inducing effects. The samples for the middle and the lower sections displayed lower activity. They also worked in a dose-dependent manner. The cells differentiation inducing rates reached 91. 38%,80. 29% and 76. 52% after treated with the upper,middle and lower section samples separately at a concentration of 800 mg m L^-1. The difference of cell differentiation inducing activity between the middle and the lower section samples was small.