醒脑液对PAP双转基因痴呆小鼠行为学及脑内Aβ_(40)、Aβ_(42)的干预作用

Early Intervention of Xingnaoye Decoction on Behavioral Science and AΒ_(40) and AΒ_(42) in Brain towards PAP Double Transgenic Dementia Mice

目的:研究醒脑液早期干预对阿尔茨海默病模型APPswe/PS△E9双转基因小鼠行为学及脑内淀粉样蛋白代谢的影响。方法:将75只PAP双转基因小鼠随机随机分为空白对照组(模型组)、阳性对照组、用药高剂量组、用药中剂量组和用药低剂量组,每组各15只。15只遗传背景相同的转基因阴性的小鼠作为正常动物组。所试药物稀释至相同体积灌胃给药,正常动物组及空白对照组给以等体积蒸馏水灌胃,连续灌胃4个月。采用Morris水迷宫测定小鼠空间学习记忆能力,避暗箱测定其条件学习记忆能力。采用ELISA法测定淀粉样蛋白40、42(Aβ40、Aβ42)的表达。结果:与正常组比较,模型组小鼠的学习记忆能力均有所下降,同时大脑组织内Aβ40、Aβ42的含量均增加。与模型组比较,阳性对照组、用药高剂量组及用药中剂量组小鼠大脑组织内Aβ40含量下降。结论:醒脑液可以干预淀粉样蛋白的代谢过程,从而减少脑内毒性蛋白的产生,进一步改善阿尔茨海默病模型小鼠的学习记忆能力。

Objective： To research the early intervention effect of Xingnaoye Decoction on behavioral science and amyloid protein metabolism in brain towards Alzheimer＇s disease model APPswe/ PS △E9 double transgenic mice. Method： 75 PAP double transgenic mice were randomly divided into blank control group（ model group）,positive control group,high dose group,medium dose group and low dose group,with 15 mice in each group. 15 transgenic negative mice of the same genetic background were as the normal control group. The Xingnaoye decoction was diluted to the same volume for three dose groups,and normal control group and blank control group were given an equal volume of distilled water,orally for 4months. The Morris water maze was used to determine learning and memory ability of mice in space,and avoid black-box to determine one in condition. We determined the expressions of amyloid protein 40,42（ Aβ 40,Aβ 42） by ELISA method. Result： Compared with the normal group,the learning and memory ability was decreased and the contents of Aβ40and Aβ42in brain were increased in model group mice. Compared with the model group,the contents of Aβ40in brain were decreased in positive control group,high dose group and medium dose group. Conclusion： Xingnaoye decoction can interfere with amyloid protein metabolism,thereby it can reduce the content of noxious protein in brain,and further improve the ability of learning and memory in mice model of Alzheimer＇s disease.