赤芍显微特征常数测定与研究

Determination and Study on Microscopic Characteristics of Radix Paeoniae Rubra

目的:利用显微定量法,对赤芍簇晶进行显微定量研究;对赤芍显微特征常数进行测定研究。方法:1容量分析法:将药材粉碎,过筛,精称定量的粉末,放入干净的研钵中,用水合氯醛试液将粉末转移至25 m L容量瓶,加入相应的甘油量,用水合氯醛定容,得供试品混悬液。充分摇匀后,用微量移液器准确吸取0.02 m L样品液置载玻片上,盖上盖玻片,完成制片。在显微镜下按"之"字形观察,记录每张片子的显微特征个数,每个样品平行做3次,每次平行装片50张,将所得结果随机分为5组,计算每组的平均值,再按照公式计算显微特征常数值。2成药前处理方法:将成药小心粉碎至完全过一定目数的筛,加适量水,充分研磨搅匀,离心,倾出上层液,向沉淀中加入清水,将沉淀搅起与水混匀,离心,不断重复上述操作,直至上层液澄清,将沉淀取出,置烘箱内低温干燥(<60℃),待干透后再将其研磨过相同目数的筛,备用。

Objective： Using microscopic quantitative method of pollen grains of saffron for microscopic quantitative study to study the microscopic characteristics of Radix Paeoniae Rubra kaempferol levels constant and correlation analysis. Methods： 1Capacity Analysis： Shatter medical materials,sieve them completely and mix together. And then weigh certain amount of drug powder accurately and put it in a clean mortar for several times with little chloral hydrate. Transfer to volumetric flask of 25 m L,clean the mortar for several times with chloral hydrate,and then unite into the same volumetric flask. Put propotional amount of glycerine,meter the volume with chloral hydrate,and then the sample was completed.Take the sample of 0. 02 m L with micro syringe after shaking sufficiently and then mount it. It was not allowed to let the liquids out and the air bubble appear while the cover glass was put on. Observe in the microscope by the shape of Chinese character Zhi and the visual field must be observed fully and not be counted repeatedly. Record the number of microscopic characteristics and do the same experiment for 3 times for each sample and mount for 50 pieces each time. Divide the results into 5 groups at random and compute the average values for each group and the microscopic characteristic constants. 2Pre- processing method of patent medicine： Shatter the patent medicine carefully and sieve by 100 meshes completely. Add proper amount of water,grind and homogenizate thoroughly,centrifuge them and remove the above liquid.Add clean water again,repeat for several times until the upper liquid was clear. Take the precipitation out after centrifugation,put it over the watch- glass and dry it below 60 ℃. Grind it and sieve by 100 meshes after dried thoroughly,then it was ready for usage.