高效液相色谱法测定磷酸肌酸钠及其有关物质

Determination of Sodium Phosphocreatine and its Related Substances by HPLC

采用高效液相色谱法测定磷酸肌酸钠及其有关物质的含量，色谱柱为phenomenex Luna HILIC，乙腈-20 mmol·L^-1磷酸氢二铵（pH为7．0）（77∶23）为流动相，流速为0．8 mL·min^-1，检测波长为210 nm，柱温为35℃．结果表明：磷酸肌酸钠与其有关物质肌酸、肌酐在同一检测条件下能够完全分离，磷酸肌酸钠、肌酸、肌酐分别在0．06～2．24（r＝0．9999）g·L^-1、0．05～2．10（r＝0．9999）g·L^-1和0．05～2．04（r＝0．9999）g·L^-1范围内浓度与峰面积呈良好线性关系，其平均回收率分别为磷酸肌酸钠97．8％，肌酸100．4％，肌酐96．1％，RSD分别为1．5％、1．3％、0．7％．该方法操作简便，灵敏度高，专属性强，可用于磷酸肌酸钠及其有关物质的测定．

A method for determination of Sodium phosphocreatine and its related substances was established by HPLC method. The determination was performed on phenomenex Luna HILIC column, acetonitrile-20 mmol/L diam-monium phosphate solution （pH 7. 0） （77∶23） as the mobile phase at a flow rate 0. 8 mL/min. The detection wave-length was 210 nm and the column temperature was 35 ℃. The results showed that sodium phosphocreatine and its re-lated substances could be completely separated under the same test conditions. Good linear relationship between the peak areas and the concentrations of sodium phosphocreatine, creatine and creatinine were 0. 06-2. 24 （r=0. 999 9） g/L, 0. 05-2. 10 （r=0. 999 9） g/L, and 0. 05-2. 04 （r=0. 999 9） g/L, respectively. The average recoveries were 97. 8%（RSD=1. 5%）, 100. 4%（RSD=1. 3%）, 96. 1%（RSD=0. 7%） respectively. The method was simple, high sensitive, good specialization, and could be used to determine sodium phosphocreatine and related substances.