塞来昔布对人肺腺癌增殖及凋亡的影响

The effects of celecoxib on proliferation and apoptosis of human lung adenocarcinoma

目的探讨选择性环氧合酶-2抑制剂塞来昔布在人肺腺癌A549细胞株增殖和凋亡中的作用及其机制。方法应用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法分析1、5和10μmol·L-1塞来昔布对A549细胞分别处理0、12、24、48和72 h后的增殖能力,分光光度法检测Caspase-3和Caspase-9活性,用免疫荧光法检测凋亡标志分子Annexin V的表达。结果塞来昔布处理组细胞的增殖能力明显低于未处理对照组细胞,差异有统计学意义(P<0.05),且抑制作用呈时间和剂量依赖性;处理组癌细胞Caspase-3、Caspase-9和Annexin V的表达水平显著高于未处理对照组(P<0.05)。结论塞来昔布可抑制人肺腺癌A549细胞的增殖并诱导凋亡,可作为肺癌患者靶向性预防和治疗药物。

Objective To study the anti - tumor functions and it＇s involving mechanisms of celecoxib on human lung adenocarei- noma cell line A549. Methods The A549 cells were treated with different duration and concentrations of celecoxib. MTT assay was used to detect the capability of cell proliferation. The process of apoptosis was determined by detecting the activity of caspase - 3 and caspase - 9 and the expression of annexin V. Results Celecoxib significantly inhibited the proliferation of A549 cells with a time and dose de- pendent manner （ P 〈 0.05 ）. Compared with untreated group, strikingly higher protein level of caspase3, caspase9 and annexin V in the celecoxib treated group were detected （ P 〈 0.05 ）. Conclusion Celecoxib can inhibit the proliferation of A549 cells and induce apopto- sis, suggesting celeeoxib might to be a good choice for human lung cancer therapy.