樟芝发酵液和菌丝体提取物抗氧化性能研究

Antioxidant Properties of Extracts from Antrodia camphorata Fermented Broth & Mycelia

采用甲醇、乙醇、异丙醇对樟芝发酵液和菌丝体进行提取,对其不同提取物进行还原力,DPPH、羟基自由基、超氧自由基、过氧化氢清除能力,亚铁离子螯合能力等抗氧化能力的测定。比较发酵液提取物和菌丝体提取物的抗氧化效果,比较不同提取剂提取物的抗氧化效果。结果显示:(1)发酵液提取物还原力最高的为异丙醇提取物,亚铁离子螯合能力最强的为乙醇提取物;DPPH清除能力乙醇和异丙醇提取物最高都达到97.88%;羟基自由基清除能力最高的为乙醇提取物,达到84.22%;3种提取物的超氧自由基清除能力都较高,具有最高清除率的异丙醇提取物达到94.89%;过氧化氢清除能力最高的为乙醇提取物的97.53%。(2)菌丝体提取物还原力最高和亚铁离子螯合能力最强的均为乙醇提取物;DPPH清除能力最高的为乙醇提取物的89.95%;3种提取物的羟基自由基清除能力普遍偏低,最高的仅为异丙醇提取物的37.28%;超氧自由基清除能力则普遍较高,最高为甲醇提取物的90.05%;过氧化氢清除能力最高的为乙醇和异丙醇提取物,最高清除率均为95.06%。抗氧化性能的研究比较,为进一步开发利用樟芝提供了依据。

Different extracts obtained by methanol,ethanol and isopropanol from Antrodia camphorata fermented broth and mycelia,their reducing power,scavenging effect on DPPH free radicals （1,1-diphenyl-2-picrylhydrazyl radicals）,hydroxyl free radicals,superoxide free radicals,hydrogen peroxide and chelating capability on ferrous ions were tested.The antioxidant effects of different extracts from broth and mycelia were compared,the antioxidant effects of extracts by different extractants were also compared.The result showed that：（1） the highest reducing power among the extracts was the one with isopropanol,the highest chelating capability of ferrous ions among the extracts was the one with ethanol,scavenging effects on DPPH by extracts with methanol and isopropanol extract had the highest and reached as high as 97.88％ ; the highest scavenging power on hydroxyl free radicals was the one extracted by ethanol,it reached as high as 84.22％.These three extracts all had high superoxide free radicals scavenging ability and the highest one was the one extracted by isopropanol it reached as high as 94.89％.The highest hydrogen peroxide scavenging ability was by the one with ethanol it reached as high as 97.53％.（2） Among the extracts of mycelia,the ethanol extract had the highest reducing power and ferrous iron chelating ability.The highest DPPH scavenging ability of ethanol extract reached 89.95％.The hydroxyl free radicals scavenging ability of these three extracts were all low,the highest one was only isopropanol extract,the scavenging ability was just 37.28％ ; while the superoxide free radicals scavenging ability were generally higher,and the highest one was methanol extract and reached up to 90.05％.The ethanol extract and isopropanol extract both had the highest hydrogen peroxide scavenging ability,the highest ratio were both 95.06％.The comparison studies on antioxidant performance provided a basis for further development and utilization of Antrodia camphorata.