HPLC同时测定大腹皮中4种生物碱含量

HPLC determination of 4 alkaloids in Arecae Pericarpium

目的:建立同时测定大腹皮中4种生物碱含量的HPLC方法,为控制大腹皮及其制剂质量提供依据。方法:采用Thermo SCX色谱柱(250mm×4.6mm,5μm);乙腈-0.1%磷酸溶液(浓氨试液调节pH值为3.3)(7327)为流动相,等度洗脱;检测波长215nm;流速1.0mL/min;柱温30℃。结果:上述色谱条件下,4种成分分离较好,在一定范围内,氢溴酸槟榔碱、盐酸槟榔次碱、氢溴酸去甲槟榔碱、盐酸去甲槟榔次碱线性关系良好,加样回收率分别为96.9%、103.0%、102.8%、102.3%,RSD分别为1.82%、1.45%、1.75%、1.12%。结论:本法准确、简便,重复性好,可用于大腹皮中4种生物碱含量测定,可作为大腹皮及其制剂多指标质量评价和控制体系的参考依据。

Objective： To determine the content of arecoline, arecaidine, guvacoline and guvacine in Arecae Pericarpium, a simple and rapid HPLC method has been developed. Methods： The separations were carried out at 30℃ on a cation-exchange column （SCX, particle size 5μm, 250mm×4.6mm）, eluted with the mobile phase consisted of acetonitril-0.1% phosphoric acid solution （adjusted to pH 3.3 with ammonium hydroxide）. The flow-rate was 1.0mL/min and the eluent was monitored at 215nm. Results： Arecoline hydrobromide, arecaidine hydrochloride, guvacoline hydrobromide and guvacine hydrochloride were well separated by this method, and showed a good linearity in the given ranges respectively. The average recoveries of the four alkaloids were 96.9%, 103.0%, 102.8%, 102.3%, RSD of them were 1.82%, 1.45%, 1.75% and 1.12% respectively. Conclusion： The present method is accurate, handy and highly repeatable, can be used to determine the contents of the 4 major alkaloids in Arecae Pericarpium, which provides a scientific basis for the multi-index evaluation and quality control system of Arecae Pericarpium and its preparation.