柚皮苷环境下微应变对成骨细胞增殖与分化的影响

Effects of the microstrain on proliferation and differentiation of osteoblast under naringin environment

目的:探讨柚皮苷环境下,微应变对大鼠成骨细胞增殖与分化的影响。方法:体外培养大鼠成骨细胞,在柚皮苷环境下,采用EF3200力学试验仪搭载的BioDynamic生物反应仓及自行研制的硅橡胶膜,对细胞进行循环牵张微应变加载。流式细胞仪检测细胞周期,RT-PCR检测骨钙素(OCN)、I型胶原(ColⅠ)及成骨特异性转录因子(Runx2)基因表达。结果:单纯力学刺激及柚皮苷联合力学刺激均能促进细胞增殖(P<0.05)。柚皮苷及单纯力学刺激均抑制细胞OCN基因表达(P<0.05)。但柚皮苷联合10%的微应变环境明显增强OCN基因表达(P<0.05)。单纯5%微应变刺激能增强Runx2基因表达(P<0.05);而柚皮苷联合力学刺激对Runx2基因表达没有影响;单纯10%微应变刺激与柚皮苷联合10%微应变刺激均增强ColⅠ基因表达。结论:微应变环境能促进成骨细胞增殖;柚皮苷环境下微应变加载能促进成骨细胞的分化功能。

Objective： To explore the effects of microstrain on the proliferation and differentiation of rat osteoblasts under naringin environment. Methods： EF3200 mechanical testing instrument with BioDynamic bioreactor system and silicone rubber membrane were used to load cyclic stretch micorstrain of rat osteoblasts under nafingin environment. Cell cycle of osteoblast was detected by flow cytometry. RT-PCR was used to detect the gene expression of osteocalcin （CON）, collagen I （Col I） and Runx2. Results： The pure mechanical stimulation and naringin combined with mechanical stimulation could promote the proliferation of cells. The pure naringin environment as well as pure mechanical stimulated environment could inhibit OCN gene expression in cells. Naringin combined with 10% microstrain environment enhanced the OCN gene expression. The pure 5% microstrain stimulation enhanced the Runx2 gene expression. The naringin enviroment combined with mechanical stimulation did not impact the Runx2 gene expression. Conclusion： The microstrain environment can promote proliferation of osteoblast. The naringin environment combines with microstrain can promote differentiation of osteoblast.