东方百合‘Sorbonne’鳞片离体培养研究

Study on Bulb Scale Culture in Vitro of Lilium oriental Hybrids 'Sorbonne'

以东方百合品种‘Sorbonne’鳞片为外植体,研究不同因子对‘Sorbonne’消毒、诱导、增殖、生根等的影响。结果表明：‘Sorbonne’外层鳞片消毒最为困难,而中层和内层差异不大；最佳消毒方法是外层鳞片用75%酒精处理30 s再用0.1%升汞浸泡18～20 min；中层和内层鳞片用75%酒精处理30 s,再用0.1%升汞浸泡14～16 min,其中不同部位鳞片诱导率从高到低分别为外层跃中层跃内层,其中外层的诱导率为76.7%；百合鳞片最佳增殖培养基为MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L,增殖系数为6.8；最佳生根培养基为1/2MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L,平均生根数为20条。

The bulb scales of Lilium oriental Hybrids ’Sorbonne’ were used as explants for in vitro culture. The results showed that the best disinfection method of the outer bulb scale explants of ’Sorbonne’ was 75% ethanol for 30 s,followed by 0.1% HgCl2 18~20 min,and the best disinfection method of the middle scale and the inner scale bulb scale explants of’Sorbonne’was 75%ethanol for 30 s,followed by 0.1%HgCl2 14~16 min. The most difficult was the outer scale disinfection,but difference of disinfection effect of the middle scale and the inner scale was smaller. The scales in different parts had different rate of induction of bulbs as followsthe outer scales＆gt;the middle scales＆gt;the inner scales. The induction rate of the outer scales was 76.7%. The appropriate medium for multiplication was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L with the multiplication coefficient of 6.8;the best medium for root induction was 1/2MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L and the number of roots per plantlets of 20.