摘要
目的:研究瘦素基因-2548A/G和瘦素受体基因Gln223Arg位点多态性与哮喘和代谢综合征的关系。方法:选取82例哮喘合并代谢综合征病人(A+M)、114例哮喘病人(AS)、100例代谢综合征病人(MS)以及96例健康对照者(NC)。根据肺功能将AS组分为轻度AS和中重度AS,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法分析所有受试者瘦素基因-2548A/G和瘦素受体基因Gln223Arg位点单核苷酸多态性及基因型,并比较不同组之间这两个基因位点和多态性之间的差异。结果:1不同组间生化指标有差异(P均<0.05);2瘦素基因-2548A/G位点多态性:MS、A+M和中重度AS组与NC相比AA基因型及A等位基因频率显著升高(P均<0.05);3瘦素受体基因Gln223Arg位点多态性:MS与NC和AS相比AG+AA基因型及A等位基因频率显著升高(P均<0.05),A+M与AS相比A等位基因频率显著升高(P<0.05),AS组、轻度AS组和中重度AS组与NC组相比均没有差异(P均>0.05)。结论:瘦素基因-2548A/G多态性与哮喘和代谢综合征都有一定的相关性,其A等位基因可能是代谢综合征的致病基因,而且与哮喘严重程度相关;瘦素受体基因Gln223Arg A等位基因可能是代谢综合征的致病基因,却与哮喘无关。
Objective:To investigate the relationship between polymorphism of leptin gene -2548A/G and leptin receptor gene (LEPR) Gln223Arg and asthma and metabolic syndrome. Methods: 82 asthma-combined metabolic syndrome patients, 114 asthma patients, 100 metabolic syndrome patients and 96 normal controls were conducted. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis were performed to investigate the polymorphism of leptin gene -2548A/G and LEPR gene Gln223Arg site. In addition, asthma was graded into mild AS and mod-severe AS according to lung function. Then the associations between the polymorphism of leptin gene -2548A/G and LEPR gene G1n223Arg and different grades of asthma were performed. Results:①The biochemical indicators were different compared between each group. ②The genotype and allele frequencies in leptin gene polymorphism -2548A/G were significantly difference between metabolic syndrome patients (P = 0. 047 and 0. 046 ) , asthma-combined metabolic syndrome patients(P=0. 038 and 0. 044) ,rood-severe asthma patients(P=O. 019 and 0. 028) and control group. ③There was a significant difference of genotype and allele frequencies in LEPR gene Gln223Arg between metabolic syndrome patients and controls (P = 0. 037 and 0. 023 ) ; between metabolic syndrome patients and asthma patients (P = 0. 000 and 0. 000 ). There was a significant difference of allele frequencies in LEPR gene Gln223Arg between asthma-combined metabolic syndrome patients and asthma(P=0. 032) . Conclusion: Polymorphisms of the leptin gene -2548A/G site may be associated with metabolic syndrome and mod-severe asthma. Polymorphisms of the LEPR gene Gln223Arg site may be only associated with metabolic syndrome. The two genes would be the candidate genes in early prevention and control.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第11期1467-1471,1476,共6页
Chinese Journal of Immunology
基金
青岛市科技局资助项目[No.11-2-4-(2)-jch]
关键词
瘦素
瘦素受体
基因多态性
哮喘
代谢综合征
Leptin
Leptin receptor
Gene polymorphism
Asthma
Metabolism syndrome