载脂蛋白E基因启动子区多态性分布在新疆哈汉民族冠心病患者之间差异性研究

Difference of Apolipoprotein E promoter polymorphism distribution between Kazak and Han patients with coronary heart disease in Xinjiang

目的:研究新疆地区哈、汉民族冠心病患者载脂蛋白E基因启动子区rs405509(G-T)、rs449647(A-T)、rs7259620(G-A)位点多态性的分布在两民族之间是否存在差异。方法:纳入病例201例,利用酚-氯仿法抽提提取DNA,再将PCR产物进行纯化。SNaPshot多重单碱基延伸反应纯化后得到的延伸产物在ABI3130XL基因分析仪上进行测序。结果:新疆地区哈萨克族与汉族患者中载脂蛋白E基因启动子区rs449647(A-T)位点基因型及等位基因在两民族之间差别具有统计学意义(P<0.05),rs405509(G-T)位点及rs7259620(G-A)位点基因型及等位基因在两民族之间差异均无统计学意义(P>0.05)。结论:载脂蛋白E启动子区rs449647(A-T)位点基因型及等位基因多态性分布在新疆地区哈、汉冠心病病人中有统计学差异,其余两个位点多态性在哈、汉民族间的分布无统计学差异。

Objective： To study the Xinjiang Kazakh,Han nationality patients with coronary heart disease apolipoprotein E gene promoter region rs405509（ G-T）,rs449647（ A-T）,rs7259620（ G-A） whether there exist differences in the two national distribution between loci polymorphism. Methods： 201 cases were studied. DNA product was extracted by using the PheNol-chloroform method and the PCR outcome was purified. We take advantage of multiple single base extension reaction to make DNA Sequencing on The ABI3130 XL. Results： The Kazak and Han patients in Xinjiang area in the apolipoprotein E gene promoter rs449647（ A-T） was statistically significant differences in genotype and allele in two ethnic groups（ P〈0. 05）,rs405509（ G-T） loci and rs7259620（ G-A） genotype and alleles in two ethnic groups had no difference statistical significance（ P〉0. 05）. Conclusion： Apolipoprotein E promoter rs449647（ A-T） genotype and allele polymorphism have significant differences between Han nationality and Kazak nationality in Xinjiang,others have no statistic difference between the two ethnic groups.