AMF诱导玉米纹枯病抗性的生理机制研究

Study on Physiological Mechanism of Maize Rhizoctonia solani Resistance Induced by Arbuscular mycorrhizal Fungi

为了探明AMF诱导玉米纹枯病抗性的生理机理,以玉米云瑞47为对象,通过盆栽试验研究了预先接种摩西球囊霉对玉米纹枯病抗性的影响,并揭示了其提高玉米抗病性的生理机制。结果表明:接种AMF能降低玉米纹枯病的病情指数;与对照相比,AMF能不同程度地提高玉米根系防御酶(POD、SOD、CAT、几丁质酶和β-1,3葡聚糖酶)的活性,在个别时期个别指标差异显著;菌根化的玉米植株根系在受到病原菌侵染时,亦能通过提高5种防御酶活性来增强抗性;同时,当纹枯病病原菌侵染后,玉米AMF侵染率及菌丝酶活性明显下降,说明两者之间存在一定的竞争关系。因此,AMF诱导玉米纹枯病的抗性与其根系的防御酶活性密切相关。

In order to reveal the physiological mechanism of Rhizoctonia solani resistance induced by AMF,taking maize variety Yunrui 47 as material,the effect of inoculating Glomus mosseae on the resistance of maize was studied in pot experiment,and the physiological mechanism was revealed.The results showed that the pre-inoculating AMF could decrease the disease index of maize.Compared with CK,the activity of five defensive enzymes（CAT,POD,SOD,chitinase andβ-1,3glucanase）of maize roots were improved variously by AMF.A single index showed significant difference in certain period.AMF colonized maize roots and also improved resistance to pathogens invasion by improving activity of the five defensive enzymes.Meanwhile,mycorrhizal colonization and AMF hyphae enzymes activity decreased obviously after pathogens invasion,that indicated certain competition relationship existed between them.Therefore,AMF induced resistance was related to defensive enzymes activity.