瓜类嫁接亲和性研究

Study on Grafting Affinity of Melon

为探索瓜类嫁接亲和力,以中国南瓜57自嫁苗、黄瓜品种津春四号自嫁苗以及津春四号/中国南瓜57嫁接苗为材料,观察嫁接后不同时期愈合部位组织结构并进行基因差异表达分析。结果表明:津春四号黄瓜自嫁苗的愈合速度快于中国南瓜57自嫁苗以及津春四号/中国南瓜57嫁接苗。不同时期南瓜自嫁苗愈合部位的cDNA-AFLP结果分析表明,特异性带均出现在嫁接后第6天取样的材料中。

In order to study the mechanism of grafting compatibility,taking self-grafted Chinese pumpkin 57,self-grafted Jinchun 4and grafted seedling Jinchun 4/Chinese pumpkin 57 as test materials,organization structure of local tissue at different stages after grafting was observed and gene differential expression was analyzed.The results showed that healing speed of self-grafted seedlings Jinchun 4was faster than others.The result of cDNA-AFLP of pumpkin self grafted seedling at different stages showed that the specific bands would appear in samples after grafting for 6days.