西瓜翠衣抑制酪氨酸酶、抗自由基作用及其成分的含量测定

Anti-tyrosinase and Antioxidation Activities and Contents Determining from Watermelon Peel

评价西瓜翠衣的抑制酪氨酸酶和抗自由基作用,并测定其成分的含量。采用酪氨酸酶活性抑制率和DPPH自由基清除率活性测定方法,分析西瓜翠衣提取物的多糖、总氨基酸和VC含量。西瓜翠衣用95%乙醇作为溶剂,提取物活性较高,其多糖、总氨基酸和VC含量分别为50.10、2.59、26.75 mg/g;提取物经大孔树脂纯化后,水洗脱部位的活性较好且富集量最大;进一步透析水洗脱部位,其透析液的酪氨酸酶活性抑制率达到58.12%,与0.25 mmol/L曲酸作用相当,DPPH自由基清除率达到56.82%。通过此研究确定了西瓜翠衣的抑制酪氨酸酶和抗自由基功效及其成分的含量。

To determine the contents and evaluate the anti-tyrosinase and antioxidation activities of extract from watermelon peel. Watermelon peel was extracted by different extraction solvents, macroporous resin purification as well as dialysis bag. And the contents of polysaccharides, amino acids, vitamine C were measured. In order to evaluate and compare the anti-tyrosinase and antioxidation activities of watermelon peel extracts , the tyrosinase activity inhibition rate and DPPH free radical scavenging rate were measured in vitro. It showed that selecting 95%ethanol as the extraction solvent, the anti-tyrosinase and anti-oxidative actions of extract were better than other solvents, in which the contents of polysaccharides, amino acids, vitamine C were 50.10, 2.59 and 26.75 mg/g, respectively. After purification by macroporous resin, water-eluting location obtained much amount and showed higher activities. The elution was further dialyzed and the tyrosinase activity inhibition rate and DPPH free radical scavenging rate of the concentrate in the outside of dialysis bag were 58.12%and 56.82%, respectively. Through the process, the anti-tyrosinase and antioxidation activities of watermelon peel was clear and the contents of active ingredients were determined.