食源性致病菌多重PCR快速检测研究进展

Research of Multiplex PCR Fast Detection on Food-borne Bacterial Pathogens

食品安全问题现在备受关注,食品中致病菌的污染造成的食物中毒事件时常发生,传统的食源性致病菌检测方法操作繁琐,耗时长,未能及时检验出食品中的致病菌,这些缺点限制了此方法的广泛应用。聚合酶链式反应(PCR)满足了快速检测致病菌的要求。主要介绍多重PCR快速检测食源性致病菌的原理、特点、检测体系的组成及其应用。多重PCR具有特异性强、灵敏度高、操作简便、可同时检测多种致病菌的优点,同时多重PCR技术可结合荧光定量PCR、基因芯片技术等建立一个更加完善的体系。

Food safety has already been become the focus of attention. Food poisoning incidents have occurred by the pollution of food-borne bacterial pathogens frequently. It is quite complex and cost much time of traditional methods for detection of food-borne pathogens, so it can＇t checkout the pathogenic bacteria in food timely. All of these disadvantages limit its application. Multiplex-PCR can meet the requirement of checking out the pathogenic bacteria fast. This article introduced the theory, characteristic, composition of detection system and application of Multiplex-PCR. Multiplex-PCR had the advantages of high specificity, high sensitivity, simple operation and checking multiple pathogenic bacteria at the same time. Furthermore, multiplex-PCR can combine with fluorescence quantitative PCR or gene chip technology to establish a better system.