均匀设计法优化超声提取泰山赤灵芝多糖工艺

Ultrasound-assisted Extraction Technology of Ganoderma lucidum Polysaccharides

试验采用超声波技术提高泰山赤灵芝子实体多糖的提取率。利用单因素试验和均匀设计试验,考察料液比、提取时间、提取温度对多糖提取率的影响,并确定最佳提取工艺条件。结果表明,影响超声辅助提取泰山赤灵芝多糖的主次因素为:提取温度>提取时间>水料比;最佳工艺条件为水料比23 m L:g,提取时间40 min,提取温度58℃,在该工艺条件下泰山赤灵芝子实体多糖提取率达2.13%。试验同时证明,均匀设计用于研究超声辅助提取泰山赤灵芝多糖工艺条件是可行的。

The experiment adopted ultrasonic-assisted technology to improve extraction rate of Ganoderma Lucidum polysaccharides. In order to study ratio of liquid to raw material, extraction time, extraction temperature on extraction rate of polysaccharides, and obtain the optional technology conditions of extraction rate of polysaccharides, the single-factor test and uniform design experiment were done. The results show that the influencing level of every factors during extracting Ganoderma Lucidum polysaccharides is as follows：extraction temperature〉extraction time〉ratio of liquid to raw material. The optimum extraction conditions were as follows：ratio of liquid to raw material was 16 mL∶g, extraction time was 40 min, extraction temperature was 58℃. The extraction rate of Ganoderma Lucidum polysaccharides was 2.13%under the optimal extraction conditions. And it＇s feasible that uniform design was used to study the ultrasound-assisted extraction Conditions of Ganoderma lucidum polysaccharides.