电感耦合等离子体质谱法检测和鉴定细菌

Detection and Identification of Bacteria Using an Inorganic Fingerprint Approach Based on ICP-MS Measurements

无机盐是细菌生长过程中不可或缺的要素,这些无机元素可在细菌体内富集,通过研究不同细菌体内不同金属元素的富集程度可能起到快速检测、鉴定细菌的目的。本研究采用ICP-MS分析了大肠杆菌(E.coli)、金黄色葡萄球菌(S.aureus)、恶臭假单胞菌(P.putida)、绿脓杆菌(P.aeruginosa)、鹑鸡肠球菌(E.gallinarum)、血液类芽孢杆菌(P.sanguinis)等6种细菌体内26种金属元素的富集程度,对其无机元素的指纹谱图进行了分析,结果表明不同细菌的无机元素浓度分布有较大区别。这种分析方法可能在细菌的检测和鉴定方面存在潜在的应用价值。

Inorganic salt offers nutrients for bacteria growth. Analyzation of the enrichment of inorganic elements of different bacteria may help to identify them. In this study, twenty-three different inorganic elements of six bacteria （E. coli, S. aureus, P. putida, P. aeruginosa, E.gallinarum and P.sanguinis） were analyzed by ICP-MS. Significant differences can be found when comparing inorganic chemical fingerprints of these bacteria. This work represents a new method toward expanding the technologies potentially in bacteria detection and identification.