液相色谱-原子荧光法测定鱼肉中有机汞形态

Determination of Organic Mercury Speciation in Fish by LC-AFS

建立微波辅助萃取-液相色谱-原子荧光（MAE-LC-AFS）测定鱼肉中甲基汞和乙基汞的分析方法。优化了液相色谱流动相、微博辅助萃取技术的提取剂浓度和萃取温度。在0～50μg/L范围内甲基汞和乙基汞的线性关系良好，线性相关系数大于0.999，甲基汞和乙基汞检出限（S/N=3）分别为0.07、0.13μg/L。3种样品在0.25、0.5、1.0 mg/kg 3个加标水平下的平均回收率为甲基汞80.3%～91.4%，乙基汞72.2%～83.3%，相对标准偏差（RSD）分别为2.4%-5.8%、2.3%-5.5%。该法适用于鱼肉中甲基汞和乙基汞的测定。

A method for simultaneous determination of methylmercury （MeHg） and ethylmercury （EtHg） in fish was developed by Microwave-Assisted Extraction-Liquid Chromatography-Atomic Fluorescence Spectrometry （MAE-LC-AFS）. Optimized the mobile phase, microwave-assisted extraction conditions, namely the concentration of the extraction solvent, the extraction temperature. Under the optimal conditions, the calibration curves for MeHg and EtHg were linear over the concentration ranges of 0- 50 μg/L and their correlation coefficients were 0.999 1-0.999 7. The detection limits of MeHg and EtHg were 0.07 and 0.13μg/L respectively. The average recoveries of MeHg and EtHg from three samples spiked at three levels of 0.25,0.5, 1.0 mg/kg were range of 80.3%-91.4%and 72.2%-83.3%with RSDs of 2.4%-5.8%and 2.3%-5.5% , respectively. This method is feasible for analysis of MeHg and EtHg in fish.