摘要
目的探讨结直肠癌中骨膜蛋白与上皮间质转化标志物E-cadhefin、N-cad．herin和临床病理参数间的相关性。方法通过免疫组织化学染色方法检测106例结直肠癌患者癌组织和对应癌旁正常组织中骨膜蛋白、E-cadherin和N—cadherin表达情况，并对其结果进行分析。结果骨膜蛋白、E-cadherin和N-cadherin在结直肠癌组织中表达与对应癌旁正常组织相比差异有统计学意义（62／106vs21／106，X2=34．027，P〈0．05；43／106vs89／106，X2=42．480，P〈0．05；66／106vs19／106，X2=43．382，P〈0．05）。结直肠癌组织中骨膜蛋白表达水平与肿瘤分化程度、肿瘤浸润程度、淋巴结转移及远处转移有关（X2=7．752，P=0．007；X2=5．008，P=0．031；X2=10．227，P=0．002；X2=8．001，P=0．006），与E-cadherin表达呈负相关（r=-0．435，P〈0．001），与N-cadherin表达呈正相关（r=0．213，P=0．028）。结论骨膜蛋白可能通过调节上皮间质转化过程参与结直肠癌的发生发展。
Objective To investigate the correlation between the Periostin and Epithelial-Mesenchymal Transi- tion（EMT） markers（ E-cadheirn and N-cadheirn） in colorectal cancer（CRC）, and analyze the relationship be- tween the expression of Periostin and clinieopathologieal parameters. Methods The expression of Periostin, E- cadheirn and N-cadheirn in 106 cases with primary CRC tumors and corresponding normal tissues were detected by Immunohistochemistry and the results were analyzed. Results The expression of Periostin, E-cadheirn and N-cad- heirn in tumors were significantly high expression than those in corresponding normal tissues （62/106 vs 21/106, X2 = 34. 027, P 〈 0. 05 ; 43/106 vs 89/106, X2 = 42. 480, P 〈 0. 05 ; 66/106 vs 19/106, X2 = 43. 382, P 〈 0.05 ）. The expression of Periostin in tumors was associated significantly with tumor differentiation, tumor invasion, lymph node metastasis and distance metastasis（x2 = 7. 752 ,P = 0. 007; X2 = 5. 008, P = 0. 031 ; X2 = 10. 227, P = 0. 002; X： = 8. 001, P = 0. 006）. It was negatively correlated with E- cadherin expression （ r = - 0. 435, P 〈 0. 001 ） , but positively correlated with N-eadherin expression（ r = 0. 213, P = 0. 028 ）. Conclusion Periostin may promote the occurrence and development of CRC by participating in EMT program.
出处
《国际外科学杂志》
2014年第11期750-754,共5页
International Journal of Surgery
基金
上海市卫生局青年科研项目（No.2010Y078）
