胃癌组织中血管内皮生长因子C的表达与淋巴结微转移的关系

Expression of vascular endothelial growth factor-C protein and m RNA in gastric carcinoma and its relationship with lymph node micrometastases

目的检测胃癌患者血管内皮因子C(VEGF-C)蛋白及m RNA在胃癌组织中的表达情况,探讨其与胃癌淋巴结微转移的关系。方法应用免疫组织化学法及RT-PCR法检测80例胃癌组织、癌旁组织及正常组织中的VEGF-C蛋白及m RNA表达情况;免疫组化法检测淋巴结微转移情况,比较有无淋巴结微转移的VEGF-C蛋白和m RNA的表达差异,明确存在的关联性。结果VEGF-C蛋白及m RNA两者在胃癌组织中明显高于癌旁组织和正常组织(P<0.05)。结论胃癌组织中的VEGF-C蛋白及m RNA与胃癌淋巴结微转移相关,基因检测优于蛋白,可作为评价胃癌患者是否存在淋巴结微转移的优选指标。

Objective To explore the expression of vascular endothelial growth factor-C （VEGF-C） protein and mRNA in gastric cancer and its relationship with lymph node micrometastases. Methods Immunohistochemistry and RT PCR were used to detect the expression of VEGF-C protein and mRNA in gastric carcinoma tissue, adjacent tissue and normal tissue from 80 patients who suffered from gastric carcinoma. The expression level of VEGF-C protein and mRNA were compared between tissues with positive or negative lymph node micrometastases. Results The positive expression rates of VEGF-C protein and mRNA in gastric cancer tissue were significantly higher than those in adjacent tissue and normal tissue（P〈 0.05）, which had no relationship with the patients＇ age, sex, tumor size, location and distant metastasis. The expression of VEGF-C was positively correlated with lymph node metastasis（P〈 0.01）, and that of VEGF-C mRNA with micrometastasis was closer than that of protein. Conclusions VEGF-C protein and mRNA in gastric cancer tissue is correlated with lymph node micrometastases, and detection of VEGF-C mRNA is prior to detection of protein. VEGF-C mRNA can be serving as a more accurate indicator of lymph node micrometastases in the gastric cancer patients.