低氧及运动对大鼠不同脑区血管内皮生长因子表达的影响

Influence of exercise and hypoxia on vascular endothelial gronth factor expression in different regions of the cerebellum in rats

目的探讨低氧和(或)运动对大鼠脑干、小脑、前脑皮质、海马和纹状体等不同脑区血管内皮生长因子(VEGF)的表达水平及脑功能的影响。方法 60只成年雄性SD大鼠随机分为对照组、低氧安静组、常氧运动组和低氧运动组。实时荧光定量聚合酶链反应(PCR)和蛋白印迹法检测建模后大鼠不同脑区VEGF表达水平的变化,Morris水迷宫实验检测大鼠神经系统功能。结果各组大鼠神经系统功能无显著差异,低氧及低氧运动引起大鼠前脑皮质、海马和纹状体VEGF表达增高,常氧运动仅引起大鼠海马VEGF表达增高。结论低氧和(或)运动可引起大鼠不同脑区VEGF表达增加,为低氧训练的开展提供进一步的实验支持。

Objective To explore the effects of hypoxia and/or exercises on the vascular endothelial growth fac-tor（VEGF） expression in different cerebral regions （brainstem, cerebellum, the frontal cortex, hippocampus and stria-tum） and the influence on cerebral function in rats. Methods Sixty male SD rats were divided into control group, hypoxic untrained group, normoxic exercise group and hypoxic training group. Real-time PCR and Western blotting were used to determine the VEGF expression level at different cerebral regions, and the function of nervous system was detected by Morris water maze test. Results The function of nervous system was not significantly different among different groups. Hypoxia and hypoxic training yielded high levels of VEGF expression of the frontal cortex, hip-pocampus and striatum, whilst normoxic exercise elicited higher levels of VEGF expression of the hippocampus. Conclusion Hypoxia and exercise result in increased VEGF expression in different regions of the cerebellum, which has provided experimental supports for the execution of hypoxic training.