钩齿溲疏的SRAP遗传多样性分析

Genetic Diversity Analysis of Deutzia baroniana Based on SRAP Marker

运用SRAP分子标记研究了山东省3个钩齿溲疏居群的遗传多样性,结果显示,用15对引物共检测到244个位点,其中多态位点167个,物种水平多态位点百分率（PPL）68.44%,Nei’s基因多样性指数（H）0.2158,Shannon’s信息指数（I）0.3282,数据表明钩齿溲疏有较高的遗传多样性水平;居群间遗传分化系数（Gst）为0.3685,表明居群间遗传变异只占36.85%,远低于居群内遗传分化;在居群水平上,以崂山钩齿溲疏居群的遗传多样性最高,徂徕山最低;居群间遗传一致度（GI）和遗传距离（GD）变化范围分别为0.8350-0.8884和0.1184-0.1803,居群间的遗传距离与地理位置间没有直接相关性。

The genetic diversity of 3 Deutzia baroniana populations from Shandong Province was studied by sequence- related amplified polymorphism（ SRAP）.The main research results were as follows.Two hundred and forty four loci were identified with 15 SRAP primer combinations,and in which,167 were polymorphic ones.The proportion of polymorphic loci（ PPL） was 68.44%.The Nei＇s gene diversity index（ H） was0.2158,and Shannon＇s information index（ I） was 0.3282,which showed rich genetic diversity in Deutzia baroniana.The coefficient of genetic differentiation（ Gst） was 0.3685,which suggested that the variation among populations occupied only 36.85% and was much lower than that in populations.At the level of populations,Laoshan population contained the highest genetic diversity,while Culaishan population had the lowest.The Nei＇s genetic identity（ GI） and genetic distance（ GD） between populations were 0.8350 - 0.8884 and0.1184 - 0.1803 respectively,and the genetic distance between populations was not directly related to geographical location.