TREM-1在大鼠急性坏死性胰腺炎并肝损伤中的作用

Role of triggering receptor expressed on myeloid cells-1 in liver injury in rats with acute necrotizing pancreatitis

目的:观察急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肝髓样细胞触发受体-1(triggering receptor expressed on myeloid cells-1,TREM-1)基因表达,探讨其与肝损伤的关系.方法:选择健康♂SD大鼠48只,随机分为对照组(C组,n=24)和模型组(A组,n=24),以5%的牛磺胆酸钠液逆行性胰胆管注射建立成ANP大鼠模型,各组分别于造模后3、6、12、24 h分批处死,检测血清淀粉酶(amylase,AMY)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)以及可溶性TREM-1和白介素-1β(interleukin-1β,IL-1β)水平;光镜下观察胰腺、肝脏组织病理学变化并评分;实时荧光定量逆转录聚合酶链反应(real-time quantitative polymerase chain reaction,qRT-PCR)检测肝内TREM-1基因表达水平.结果:A组大鼠AMY、可溶性TREM-1、IL-1β于造模后3 h开始升高,各时点均处于高水平状态,均较同时间点C组大鼠升高(P<0.05),且A组大鼠可溶性TREM-1与胰腺病理评分呈正相关(r=0.481,P<0.05);A组大鼠血ALT、AST浓度在造模后6、12、24 h均较同时点C组升高(P<0.05);A组大鼠胰腺及肝脏病理损伤和评分均高于同时点C组(P<0.01);A组大鼠肝脏组织TREM-1 mRNA表达较C组增加(3 h:1.96±0.63 vs 0.94±0.23;6 h:4.46±1.42 vs 0.95±0.24;12 h:2.59±1.14 vs 1.10±0.33;24 h:2.56±1.08 vs 0.85±0.27;均P<0.05).结论:A组大鼠肝脏组织中TREM-1 mRNA表达以及血清可溶性TREM-1浓度均增加,TREM-1可能在ANP并肝损中发挥重要作用.

AIM： To investigate the expression of triggering receptor expressed on myeloid cells-1（TREM-1） in liver injury with acute necrotizing pancreatitis（ANP） and to explore the correlation between TREM-1 expression and liver injury in rats with ANP. METHODS： Forty-eight male Sprague-Dawley rats were randomly divided into two groups： a control group and an ANP group. ANP wasinduced by retrograde injection of 5% sodium taurocholate into the biliary pancreatic duct. Rats were sacrificed at 3, 6, 12 and 24 h after treatment. Serum amylase（AMY）, alanine ami-notransferase（ALT）, aspartate aminotransfer-ase（AST）, sTREM-1 and interleukin-1β（IL-1β） were measured. The pathologic changes of the pancreatic and hepatic tissues were observed and graded under a microscope. The expression of TREM-1 mRNA in the liver was detected by real-time quantitative（qRT） PCR. RESULTS： The levels of serum amylase, sTREM-1 and IL-1β began to increase at 3 h after sodium taurocholate injection, and were then maintained at high levels at all subse-quent time points, significantly higher than those in the control group（P 〈 0.05）. The level of sTREM-1 in the ANP group was correlated with Schmidt score（r = 0.481, P 〈 0.05）. Serum ALT and AST concentrations at 6, 12 and 24 h, the pathologic scores of pancreatic and liver tissues, and the expression of TREM-1 mRNA（3 h： 1.96 ± 0.63 vs 0.94 ± 0.23; 6 h： 4.46 ± 1.42 vs 0.95 ± 0.24; 12 h： 2.59 ± 1.14 vs 1.10 ± 0.33; 24 h： 2.56 ± 1.08 vs 0.85 ± 0.27） in the liver were sig-nificantly higher in the ANP group than in the control group（P 〈 0.05 for all）. CONCLUSION： The expression of TREM-1 mRNA in liver tissue and the level of serum sTREM-1 increase significantly in rats with ANP, suggesting that TREM-1 may play an important role in ANP with liver injury.