酒精性脂肪肝中8-OHdG与TBARS的表达及维生素E的干预实验

Expression of 8-OHdG and TBARS in alcoholic fatty liver in rats: Implications for therapeutic effects of vitamin E

目的:通过测定酒精性脂肪肝(alcoholic fatty liver,AFL)大鼠模型肝组织内甘油三酯(triglyceride,TG)、8-羟基脱氧鸟苷酸(8-hydroxy-guanin,8-OHdG)与硫代巴比妥酸反应产物(thiobarbituric acid reaction substances,TBARS)的含量及抗氧化剂维生素E(vitamin E,VE)的干预研究,探讨氧化应激(oxidative stress,OS)及脂质过氧化反应(lipid peroxidation,LPO)在AFL发病中的作用.方法:将45只Wistar大鼠随机分为空白组(Control,n=15)、模型组[(ethanol,ET),n=15]及实验组(ET+VE,n=15),空白组给予蒸馏水灌胃;AFL模型组给予鱼油2.5 mL/kg+ET灌胃;实验组采用鱼油+ET灌胃,同时加用VE 400 mg/kg灌胃.第8周取肝组织,采用比色法测定肝脏TG沉积;酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)测定肝脏8-OHdG和肝脏TBARS含量.结果:与对照组相比,AFL模型组大鼠肝脏TG沉积增加(0.72±0.09 vs 0.28±0.07,P<0.01),肝8-OHdG(1.45±0.17 vs 0.52±0.21,P<0.01)及TBARS(65.2±9.7 vs 51.2±12.9,P<0.01)含量明显升高;与模型组相比,经VE干预后,实验组大鼠肝内TG(0.53±0.11 vs 0.72±0.09,P<0.01)沉积减少,8-OHdG(0.99±0.27 vs 1.45±0.17,P<0.01)及TBARS(55.6±12.6 vs 65.2±9.7,P<0.01)含量降低.结论:AFL大鼠肝组织内TG沉积明显,肝内8-OHdG和TBARS含量明显升高,经抗氧化剂VE干预后,TG沉积减少,肝内8-OHdG和TBARS含量降低.OS损伤及LPO损伤在AFL的发病过程中具有重要作用,抗氧化剂VE可通过减轻OS及LPO损伤协同防治AFL.

AIM： To explore the role of oxidative stress（OS） and lipid peroxidation（LPO） in alcoholic fatty liver（AFL） in rats and to investigate the effect of vitamin E（VE） on AFL. METHODS： Forty-five Wistar rats were randomly divided into three groups： a control group（n = 15）, an ethanol group（ET, n = 15）, and an ET ＋ VE group（n = 15）. The control group was given dis-tilled water by gavage once a day for 8 wk. The ET group was given ET and fish oil by gavage. The ET ＋ VE group was given additional VE com-pared with the ET group. Triglyceride（TG） in the liver was measured by colorimetric method. The contents of 8-hydroxy-deoxyguanosine（8-OHdG） and thiobarbituric acid reactive substances（TBARS） in the liver were measured by enzymelinked immunosorbent assay（ELISA）. RESULTS： The ET group had a significant in-crease in TG compared with the control group（0.72 ± 0.09 vs 0.28 ± 0.07, P 〈 0.01）. After VE in-tervention, TG in the liver decreased. 8-OHdG（1.45 ± 0.17 vs 0.52 ± 0.21, P 〈 0.01） and TBARS（65.2 ± 9.7 vs 51.2 ± 12.9, P 〈 0.01） increased significantly in the ET group compared with the control group. 8-OHdG and TBARS decreased significantly in the ET ＋ VE group compared with the ET group. CONCLUSION： TG deposits obviously in the liver of AFL rats. OS and LPO play an important role in the pathogenesis of AFL. VE as an antioxi-dant can ameliorate AFL induced injury.