摘要
利用实时定量PCR的方法分析了tsa-mi R396在盐芥不同组织的表达情况及盐、冷胁迫下的表达量变化,结果显示tsami R396在盐芥的不同组织均有表达,且在盐芥幼苗受盐、冷胁迫处理2 h后其表达量明显上升,随后呈波动性变化。利用在线软件ps RNATarget预测到25个tsa-mi R396的靶基因并进行功能分类。成功克隆tsa-mi R396前体,并利用Mfold在线软件分析该前体能够折叠形成茎环结构。
In this study, we analyzed the expression levels of tsa-mi R396 in different tissues and response to the salt and cold stresses by real-time quantitative RT-PCR. The results showed that tsa-mi R396 widely expressed in different tissues of Thellungiella salsuginea and its expression level was strongly up-regulated at 2 hours of salt or cold treatment and then changed in volatility. Using the ps RNATarget online 25 target genes of tsami R396 were predicted and classifi cated according to their functions. Moreover, the precursor of tsa-mi R396 was successfully cloned and could fold into the stem-loop structure by Mfold web server.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第11期1692-1698,共7页
Plant Physiology Journal
基金
国家自然科学基金(31170368)
关键词
盐芥
MIRNA
实时定量PCR
靶基因
前体
Thellungiella salsuginea
miRNA
real-time quantitative PCR
target gene
precursor
