载布洛芬的PLGA/PLA电纺纳米纤维GTR膜的实验研究

The biological characteristics of electrospun ibuprofen-loaded PLGA/PLA nanofibrous GTR membrane: An in vitro study

目的:通过静电纺丝技术制备载不同含量布洛芬(IPF)的聚乳酸-羟基乙酸共聚物(PLGA)/聚乳酸(PLA)纳米纤维引导组织再生(GTR)膜,评价其生物学性能。方法:使用静电纺丝技术制备PLA纳米纤维膜,扫描电镜观察其表面形貌,与EMT-6细胞复合培养,检测其生物相容性及机械阻挡功能;制备载不同含量布洛芬的PLGA/PLA纳米纤维膜,观察其表面形貌、MTT法检测人牙龈成纤维细胞(HGFs)在其上的增殖情况,同时检测其载药率及药物缓释情况。结果:PLA以及载入不同量IPF的电纺纤维的直径均为300 nm^5μm,载入布洛芬后,纳米纤维仍表面光滑、均一、连续;绝大多数的EMT-6细胞均黏附在PLA膜的表面,膜内部几乎没有细胞;HGFs与不同载药量的IPF-PLG/PLA电纺膜复合培养1、3、5、7 d时,各载药实验组在各时间点的OD值与空白对照组相比P>0.05;5%、10%、15%3种载药量的电纺膜的载药率均可达85%以上,在1周内的突释量分别为30%、60%、85%,突释较小,药物释放时间可持续10~28 d。结论:本实验所制备的载布洛芬PLGA/PLA电纺纳米纤维膜具有良好生物相容性、优异的阻挡功能及缓慢释药功能。

AIM： To investigate the biological performance of ibuprofen（ IPF）- loaded electrospun poly lactic- co- glycolic acid（ PLGA） / poly lactic acid（ PLA） nanofibrous guided tissue regeneration membrane. METHODS： PLA nanofibrous membranes and IPF（ at 5%,10% and 15% respectively）- loaded PLGA / PLA（ IPF- PLGA / PLA） nanofibrous membranes were synthesized using an electrospinning technique. The morphology of the nanofibers was observed under SEM. Human gingival fibroblasts were seeded on IPF- PLGA / PLA membranes and HGFs proliferation was assessed with MTT assay. Drug entrapment efficiency was determined and drug release profile was tested. RESULTS： The diameters of the nanofibers were 300 nm- 5 μm,and the IPF- loaded nanofibers were smooth,uniform and continuous. The majority of EMT-6 cells adhered on the surface of PLA membranes,while almost no cells appeared in the membranes. MTT assay showed that the number of EMT- 6 cells of the experimental group was not significantly different from that of the control group after 1,3,5 and 7 day culture（ P 〈0. 05）. Ibuprofen entrapment efficiencies were above 85% for all the IPF- loaded membranes. The release of ibuprofen at 5% 、10% 、15%wrapped in fiber was 30%,60% and 85% respectively in 7 days and the release was going on for 10- 28 days. CONCLUSION： IPF- PLGA / PLA nanofibrous membranes are biocompatible with excellent barrier function for cells and slow drug release.