树脂充填前后唾液中变异链球菌的变化

Comparison of Streptococcus mutans in saliva before and after dental caries filling with resin-based composite

目的观察使用树脂进行龋齿治疗前后唾液中变异链球菌数量的变化。方法招募志愿者20名,对其口腔内所有龋齿去净龋坏组织后使用不含氟树脂材料进行充填,分别收集充填前及充填后第1、2、3、4周的非刺激性唾液样本,使用轻唾-杆菌肽琼脂培养基厌氧培养48 h后检测变异链球菌数量,比较其在龋齿充填治疗前后的变化。结果龋齿行树脂材料充填后1周唾液中变异链球菌数量较充填前下降明显(P<0.05),充填后2、3、4周较充填前无明显变化(P>0.05)。结论对患龋个体进行龋齿备洞后的树脂充填能在短期内降低唾液中变异链球菌的含量,但随时间推移,唾液中变异链球菌数量会逐渐恢复到治疗前的状态。单纯依靠龋齿充填治疗并不能改变唾液中变异链球菌的数量。

Objective This study was designed to compare the changes in Streptococcus mutans（S, mutans） in the saliva before and after dental caries filling with resin-based composite. Methods Twenty volunteers participated in the study. The caries in the oral cavities of the volunteers were removed, and filling with resin-based composite without fluoride was performed. Non-stimulated saliva was obtained prior to treatment as well as 1, 2, 3, and 4 weeks post treatment. Approximately 100 μL of salivary sample was transferred, plated on mitis salivarius with bacitracin agar, and incubated anaerobically at 37 ℃ for 48 h. Results Compared with the number of S. mutans in the saliva prior to treatment, the number ofS. mutans in the saliva 1 week after filling significantly decreased（P〈0.05）. However, no significant differences were found in the number of S. mutans in the saliva at 2, 3, and 4 weeks after filling（P〉0.05）. Conclusion The number of S. mutans in the saliva could be reduced for a certain period through caries removal and resin-based composite filling. However, the number of S. mutans in the saliva would gradually revert to its status prior to the treatment of dental caries. Relying only on the filling treatment of dental caries does not change the number of main cariogenic microorganisms in the saliva.