宫颈癌患者血清TSGF、SCC含量检测及肿瘤恶性生物学行为的评估

Detection of cervical cancer patients' serum TSGF,SCC content and evaluation tumor malignant biological behavior

目的:研究宫颈癌患者血清TSGF、SCC含量及肿瘤恶性生物学行为。方法:选取在本院就诊的宫颈癌患者纳入研究的观察组,将同期就诊的宫颈上皮内瘤变患者纳入研究的对照组,采集外周血检测肿瘤特异性生长因子(TSGF)、鳞状细胞癌相关抗原(SCC)含量,收集宫颈组织检测B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关的X蛋白(Bax)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的mRNA和蛋白含量。结果:(1)血清学指标:观察组的血清TSGF、SCC含量以及阳性率均高于对照组,差异有统计学意义(P<0.05);(2)凋亡分子:观察组宫颈组织中Bcl-2的mRNA和蛋白含量高于对照组,而Bax和Caspase-3的mRNA和蛋白含量低于对照组,差异有统计学意义(P<0.05);(3)侵袭分子:观察组宫颈组织中MMP-2和MMP-9的mRNA和蛋白含量高于对照组,差异有统计学意义(P<0.05)。结论:宫颈癌患者血清TSGF、SCC含量异常升高;且Bcl-2、MMP-2和MMP-9处于高表达状态,而Bax和Caspase-3处于低表达状态。

Objective：To study the serum TSGF,SCC of the patients with cervical cancer and tumor malignant biological behavior.Methods：Cervical cancer patients were enrolled in observation group;cervical intraepithelial neoplasia patients were enrolled as control group.Peripheral blood were collect and TSGF,SCC content were detected;cervical tissue were collected and mRNA and protein content of Bcl-2,Bax,Caspase-3,MMP2,MMP9 were assayed.Results：（1）Serum index：serum TSGF,SCC content and positive rate of observation were higher than those of control group;（2）Apoptosis molecule：mRNA and protein of Bcl-2in observation group patients’ cervical tissue were higher than that in control group,while mRNA and protein of Bax and Caspase-3in observation group patients’ cervical tissue were lower than those in control group;（3）Invasion molecule：mRNA and protein of MMP2 and MMP9in observation group patients’ cervical tissue were higher than those in control group.Conclusion：Cervical cancer patients’ serum TSGF,SCC levels abnormally increase;Bcl-2,MMP2 and MMP9are highly expressed,Bax and Caspase-3are in low expression state.