传统活性污泥法和膜生物反应器驯化期微生物群落组成特征

Microbial Community Characteristics of Traditional Activated Sludge Process and MBR in Acclimation Period

[目的]比较传统活性污泥法(S1)和膜生物反应器(MBR,S2)工艺微生物群落之间的差异。[方法]通过Mi Seq高通量测序平台,分析处于驯化期的S1和S2工艺中微生物群落结构组成。[结果]从S1和S2分别获得47 354和51 882条有效序列,平均长度为253bp。在97%相似性水平下,从S1和S2分别可确定2 693和3 208个操作分类单元(OTU),其中有1 156个OTU为相同单元。S1和S2的丰度指数(Chao 1)分别为6 639.3和9 564.1,Shannon指数为9.03和9.13。系统进化分析结果表明,两种处理系统中活性污泥的优势菌群均为变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、拟杆菌门(Bacteroidetes);同时,硝化螺旋菌门(Nitrospirae)在MBR中更易富集,所占比例要高于传统活性污泥法。[结论]在驯化期中,MBR和传统活性污泥法均具有较高的微生物多样性以及相似的优势菌群结构。由于对微生物的高效过滤作用,驯化期MBR中微生物群落多样性更为丰富。

[ Objective] To compare the microbial community characteristics in a traditional activated sludge process （ S1 ） and membrane bioreactor （MBR, $2）. [ Method] MiSeq high-throughput sequencing was used to investigate the microbial community characteristics in S1 and $2. [ Result] The results showed that 47 354 and 51 882 reads with an average read length of 253 bp were found from S1 and $2, respective- ly. At the similarity level of 97% , 2 693 and 3 208 operational taxonomic units （OTUs） were obtained from S1 and $2, respectively, and number of common OTUs was 1 156. The richness index （Chao 1 ） of S1 and $2 were 6 639.3 and 9 564.1, and Shannon index were 9.03 and 9.13, respectively. In addition, Analysis of system evolution demonstrated that Proteobacteria, Planctomycetes and Baeteroidetes were the dominant phylum in both S1 and $2. Morever, the nitrifying bacteria could be enriched more effectively in MBR, and the ratio of Nitrospirae was higher in comparison with that in the traditional activated sludge process, [ Conclusion] In acclimation period, both traditional activated sludge process and MBR had very high microbial community diversity, and similar microbial community structure at phylum level. Higher di- versity could be found from MBR in acclimation period due to its efficient filtration.