miR-30通过调节CD90表达抑制肝细胞肝癌

miR-30 functions as a tumor suppressor by modulating CD90 in hepatocellular carcinoma

目的:mi RNA能通过转录后调节靶基因的表达量,并在致癌过程中发挥抑癌或促癌作用。研究发现mi R-30家族在人类肿瘤中起着重要作用,并参与维持干细胞特性的上皮细胞间质化过程,本研究主要识别mi R-30家族与肝细胞肝癌的关系。方法:应用q RT-PCR实验方法检测93例肝细胞肝癌患者癌组织和癌旁正常组织中mi R-30c,mi R-30b和mi R-30e表达水平,另对121例肝细胞肝癌患者癌组织进行mi R-30家族的表达及CD90免疫组织化学表达检测,分析mi R-30家族与CD90在肝细胞肝癌中的相关性。结果:本研究发现mi R-30c(P<0.001)、mi R-30b(P=0.004)和mi R-30e(P<0.001)与癌旁正常组织相比,癌组织中表达显著下调。CD90蛋白在癌组织中的表达水平显著高于癌旁正常组织(P=0.007)。Mi R-30c(P=0.032)和mi R-30e(P=0.015)在CD90阴性表达的患者中表达水平显著高于CD90阳性表达的患者。结论:mi R-30家族在肝细胞肝癌中可作为抑癌mi RNA,并通过调节CD90蛋白来发挥这一作用。

Objective： miRNA can post-transcriptionally regulate the expression of target genes and act as oncogenes or tumor suppressor genes in tumorigenesis. Emerging evidence demonstrates that the miR-30 family may perform an important function in hu- man cancers and can regulate epithelial-mesenchymal transition, which has a critical role in cancer stem cells. This research was con- ducted to identify the possible association between the miR-30 family and hepatocellular carcinoma （HCC）. Methods： The expression of miR-30c, miR-30b, and miR-30e in 93 tumor tissues and adjacent tissues were measured by real-time reverse transcription PCR. Fur- thermore, 121 tumor tissues from an independent cohort were selected to measure the expression level of miR-30 family and CD90 by immunohistochemistry. The relationship between miR-30 family and CD90 protein expression was analyzed. Results： The expression levels of miR-30c （P〈0.001）, miR-30b （P=0.004）, and miR-30e （P〈0.001） were significantly decreased in tumor tissues compared with adjacent tissues, and the expression level of CD90 in tumor tissues was significantly higher than that in adjacent normal tissues （P= 0.007）. In addition, the expression levels of miR-30c （P=0.032） and miR-30e （p=0.015） were significantly high in negative staining for CD90 when compared with positive staining for CD90. Conclusion： Taken together, we suggest that the miR-30 family may act as a tu- mor suppressor in HCC development and may modulate CD90 protein expression.