胃癌患者循环肿瘤细胞的检测及临床意义

Detection and clinical significance of circulating tumor cells in patients with gastric cancer

目的:探讨胃癌患者外周血中循环肿瘤细胞的临床意义。方法:采集45例胃癌患者的外周血,应用密度梯度离心法和免疫荧光法检测外周血中的循环肿瘤细胞(circulating tumor cells,CTCs),并比较其临床病理意义。结果:应用密度梯度离心法和免疫荧光法检测胃癌患者外周血中的CK19阳性肿瘤细胞即为CTCs。45例胃癌患者中27例为CTCs阳性,CTCs阳性与淋巴结转移(P=0.007)、远处转移(P=0.035)和复发密切相关(P=0.035),而与性别、年龄、肿瘤部位和TNM分期、肿瘤分化程度无关(P>0.05)。结论:CTCs与胃癌的不良预后相关。

Objective： To detect circulating tumor cells （CTCs） in patients with gastric cancer and evaluate the relationship among CTCs, clinico-pathological characteristics, and prognosis of gastric cancer. Methods： Peripheral blood samples （10 mL in EDTA） were obtained from 45 patients with gastric cancer. CTCs were detected using density-gradient centrifugation and immunofluo- rescence staining. The clinical significance of the two methods were also compared and investigated. Results： CTC-positive case was defined by the presence of at least one CK19 （＋）-CTC per 10 mL of the sample. CTCs were found in 27 of the 45 patients with gastric cancer. The presence of CTCs was significantly correlated with lymph node metastasis, distant metastasis, and recurrence （P=0.007, 0.035, 0.035, respectively）. However, CTCs were not significantly correlated with sex, age, tumor location, TNM staging, and tumor differentiation （P〉0.05）. Conclusion： CTCs were associated with poor prognosis of gastric cancer.