电解蚀刻法处理的钛及钛合金表面的对比研究

Comparative study of the titanium and titanium alloy implant electrolytic etching surface

目的通过成骨细胞的体外培养,初步探讨钛及钛合金微-纳米三维形貌对成骨细胞生物学行为的影响。方法采用电解蚀刻法在纯钛及钛合金表面构建出不同尺寸的微-纳米三维形貌,并观察其三维结构表面对成骨细胞黏附、增殖、细胞形态、碱性磷酸酶（ALP）活性的影响。结果在成骨细胞的黏附和增殖方面,纯钛组和钛合金组表面均高于纯钛机械抛光组。纯钛组表面细胞胞体饱满,伸出大量伪足,并可见大量功能颗粒。ALP活性显著高于钛合金和纯钛机械抛光组表面。结论通过电解蚀刻法在纯钛和钛合金表面可形成不同直径和深度的碗形巢样及纳米结构;两个表面即30~50μm和5~8μm的表面和光滑表面相比,都明显促进了细胞的附着;30~50μm的纯钛表面更有利于促进细胞的增殖和分化。

Objective This preliminary study aims to investigate the effects of titanium and titanium alloy micro-nanodimensional topography on the biological behavior of osteoblasts in vitro.Methods Electrolytic etching（EE） method was used to produce micro-nano dimensional titanium surfaces.The surfaces were observed to determine their effects on the adhesion,proliferation,cell morphology,and alkaline phosphatase（ALP） activity of osteoblasts.Results The surfaces of the titanium and titanium alloy groups exhibited higher adhesion and proliferation of osteoblasts than those of the mechanical group.The titanium surface was covered with a group of cells,a large number of filopodia,and functional particles.The ALP activity of the titanium group was significantly higher than that of the titanium alloy and mechanical groups.Conclusion EE method in pure titanium and titanium alloy surfaces result in bowl-like nests and nanostructures of different diameters and depths.The diameters of the pure titanium and titanium alloy surfaces range from 30 to 50 μm and 5 to 8 μm,respectively.The former is more conducive to promote the proliferation and differentiation of cells.