海岛湿热作战条件下脊髓火器伤动物模型的病理观察

Establishment of Experimental Animal Model of Spinal Firearm Wounds under Conditions of High Temperature and High Humidity

目的:建立高温高湿条件下猪脊髓火器伤模型,观察受伤节段脊髓的病理变化。方法:选用6头南方实验用猪构建高温高湿条件下胸腰段脊髓高能火器伤模型,伤时及6 h后取脊髓组织观察其病理形态学改变。结果:大体观察符合高能量火器伤损伤特点,伤后镜下均可见脊髓硬脊膜血管扩张,硬膜下淤血明显,灰质中神经元细胞变性等改变,6 h后神经元消失溶解,灰质神经元尼氏体大量发生崩解。结论:采用适龄猪建立高温高湿环境下脊髓火器伤模型,造模稳定,重复性较好。

Objective：To establish the firearm injury model in the environment of high temperature and humidity and to study the pathological changes of the spinal cord after injury. Methods：Six healthy porcine were subjected to SCI models with high-energy gunshot in spinal cord of breast and lumbar section. The pathological changes of spinal cord were observed under optical microscopy （OM） immediately and six hours after surgery. Results：The general pathological changes were in agreement with high-energy ignition injury damage characteristics. Under OM, the blood vessels of spinal dura mater were dilated; subdural blood congestion were obvious, and neuronal cells in grey matter were degenerated, disappeared and dissolved;gray Nissl body mass were collpsed. Conclusion：The model was established with good stability and reproducibility.