BALB/c突变卷毛小鼠血液学指标的检测分析

Detection and analysis of hematological indexes in BALB/c mutant curly mice

目的探讨BALB/c突变卷毛小鼠的血液学指标与正常BALB/c小鼠之间是否存在突变差异。方法选择6周龄的BALB/c突变卷毛小鼠和正常BALB/c小鼠各20只(雌雄各半),使用全自动血细胞分析仪检测8项血液常规指标;使用全自动生化仪检测15项血清电解质及生化指标,并对两组结果进行对比分析。结果 BALB/c突变卷毛小鼠的白细胞、平均血细胞容积、血小板计数和平均血细胞血红蛋白浓度的性别及组间结果与正常小鼠具有显著性差异(P<0.05,P<0.01);BALB/c突变卷毛小鼠Na+、K+和Cl-的性别及组间结果与正常小鼠具有显著性差异(P<0.05,P<0.01);BALB/c突变卷毛小鼠丙氨酸转氨酶、葡萄糖和甘油三酯的性别和组间结果与正常小鼠具有显著性差异(P<0.05,P<0.01)。结论 BALB/c突变卷毛小鼠和正常BALB/c小鼠的部分血液学指标存在明显差异,这为今后研究和应用BALB/c突变卷毛小鼠模型提供了理论参考。

Objective To investigate if there are differences in hematological indexes between BALB/c mutant curly mice and normal BALB/c mice. Methods 6 week old BALB/c mutant curly and normal BALB/c mice of each 20 （male and female）were selected, and 8 blood routine indexes and 15 serum electrolytes and biochemical indexes were detected using automatic blood cell analyzer and automatic biochemical detection, and the results of two groups were compared. Results The sex and group results of WBC, MCV,PLT and MCHC between BALB/c mutant curly mice and normal mice have significant differences （ P 〈 0.05, P 〈 0.01 ） ; the sex and group results of Na^＋ , K^＋ and C1^- between BALB/c mutant curly mice and normal mice have significant differenees（P 〈 0.05,P 〈 0.01 ） ;the sex and group results of A LT, GLU and TG between BALB/c mutant curly mice and normal mice have significant differences （P 〈 0. 05,P 〈 0. 01 ）. Conclusion Some hematological indexes between BALB/c mutant curly and normal BALB/c mice are different clearly, and these results will provide the theoretical references for researching and using mutant curly mice model.