三种固定液对大、小鼠肠道组织石蜡切片质量的影响

Comparison on rat and mice intestine slides' quality fixed with three different fixatives and embeded by paraffin

目的对比三种固定液对大、小鼠肠道组织石蜡切片质量的影响,优选大、小鼠肠道组织切片固定液。方法选择三种常用的固定液:10%中性福尔马林固定液,Bouin’s固定液,改良Davidson’s固定液,对SD大鼠和KM小鼠小肠组织进行固定,常规制作石蜡切片、HE染色,对比观察其染色效果。结果三种固定液对大、小鼠肠道组织石蜡切片质量存在影响,单纯福尔马林固定的切片存在肠粘膜上皮脱落、坏死的假象;Bouin’s固定液和改良Davidson’s固定液则可避免这一假象的出现,而且切片结构更精细、清晰,但是,HE染色的色彩不如单纯福尔马林固定液的鲜艳。结论 Bouin’s固定液和改良Davidson’s固定液固定的大、小鼠肠道组织的石蜡切片质量优于10%中性福尔马林固定液。

Objective To compare the rat and mice intestine slides quality fixed with three different fixatives and embeded by paraffin. Methods Three kinds of conventional fixatives were selected for the test, they are 10% neutral buffered formalin,Bouin＇ s solution and modified Davidson＇ s solution. SD rat＇ s and KM mice＇ s intestinal tissues were fixed with the three different fixatives, then embeded, sliced and stained with HE as routine rut. To observe the slides quality under microscope. Results Sheding and necrosis of intestine villus epithelia were observed in slides made from formalin fixed intestines, which is speculated due to the fixations. The phenomenon was not observed in Bouin＇s solution and modified Davidson＇s solution fixed tissues. And the tissue structure were more fine and clear in them. Conclusions The rat and mice intestine slides made from tissues fixed by Bouin＇s solution and modified Davidson＇s solution are better than from the 10% neutral buffered formalin fixed ones.